Bauer Ilona, Hassmann-Poznańska Elzbieta, Wysocka Jolanta, Kasprzycka Edwina, Ratomski Karol
Department of Anesthesiology and Intensive Care, Medical Academy, Białystok, Poland.
Int J Pediatr Otorhinolaryngol. 2008 Mar;72(3):321-6. doi: 10.1016/j.ijporl.2007.10.020. Epub 2007 Dec 21.
The significance and protective efficacy of surface secretions on mucosal membranes in the upper airways are well recognized. The aim of our study was to reveal the role of the adenoids as a source of cellular components in the mucosal secretion.
The adenoid removed because of its hypertrophy and the samples of surface secretions taken by "imprint method" described by Ebenfelt et al. from the group of 38 children were examined by flow cytometry. The lymphocyte subsets with following antigens: CD3, CD4, CD8, CD19, CD23, CD16+56, CD45RA, CD45RO, HLA-DR were analyzed. Two groups were studied: (1) AH, children with adenoid hyperplasia without otitis media; (2) OME, children with adenoid hyperplasia and persistent otitis media with effusion. Age-related changes in percentages of lymphocyte subpopulations were analyzed.
The percentage of lymphocyte subsets in adenoid and secretion were similar, however, in the surface secretion there was higher proportion of lymphocytes B and NK and lower of T. There was no correlation between percentages of lymphocyte in adenoid and surface secretion except for lymphocytes Th with memory phenotype and lymphocytes B with marker CD23(+). In adenoid the percentage of T and Th lymphocytes increased with age and the percentage of B lymphocytes decreased. In the secretion age-related changes were less pronounced and only the percentage of lymphocytes B CD23(+) showed positive correlation with age. In cases of otitis media with effusion there was higher proportion of lymphocytes B (CD19(+) and CD19(+)CD23(+)) and lower of lymphocytes Ts and Th with naive phenotype in the secretion. In adenoid however, the cases of OME revealed lower percentage of lymphocytes B and higher of lymphocytes Ts.
Similar proportions of lymphocyte subpopulations in surface secretion and in adenoid imply that adenoid is the source of those cells. However, lack of correlation between the percentages of lymphocytes in secretion and in adenoid suggest that the process of lymphocyte migration has active and selective character.
上呼吸道黏膜表面分泌物的重要性及保护作用已得到充分认识。本研究旨在揭示腺样体作为黏膜分泌物细胞成分来源的作用。
对38名因腺样体肥大而切除的腺样体以及采用Ebenfelt等人描述的“印记法”从这些儿童中获取的表面分泌物样本进行流式细胞术检测。分析具有以下抗原的淋巴细胞亚群：CD3、CD4、CD8、CD19、CD23、CD16 + 56、CD45RA、CD45RO、HLA - DR。研究了两组：（1）AH组，腺样体增生但无中耳炎的儿童；（2）OME组，腺样体增生且伴有持续性中耳积液的儿童。分析淋巴细胞亚群百分比的年龄相关变化。
腺样体和分泌物中淋巴细胞亚群的百分比相似，然而，表面分泌物中B淋巴细胞和自然杀伤细胞（NK）的比例较高，T淋巴细胞的比例较低。除了具有记忆表型的辅助性T淋巴细胞（Th）和带有CD23(+)标记的B淋巴细胞外，腺样体和表面分泌物中淋巴细胞的百分比之间没有相关性。在腺样体中，T淋巴细胞和Th淋巴细胞的百分比随年龄增加，B淋巴细胞的百分比下降。在分泌物中，年龄相关变化不太明显，只有CD23(+)B淋巴细胞的百分比与年龄呈正相关。在中耳积液的病例中，分泌物中B淋巴细胞（CD19(+)和CD19(+)CD23(+)）的比例较高，具有幼稚表型的抑制性T淋巴细胞（Ts）和Th淋巴细胞的比例较低。然而，在腺样体中，OME病例显示B淋巴细胞的百分比较低，Ts淋巴细胞的百分比较高。
表面分泌物和腺样体中淋巴细胞亚群的比例相似，这意味着腺样体是这些细胞的来源。然而，分泌物和腺样体中淋巴细胞百分比之间缺乏相关性，表明淋巴细胞迁移过程具有活跃和选择性的特征。
