Matsuoka A, Kodama Y, Fukuhara K, Honda S, Hayashi M, Sai K, Hasebe M, Fujiwara Y
Division of Medical Devices, National Institute of Health Sciences, Tokyo, Japan.
Food Chem Toxicol. 2008 Mar;46(3):1125-30. doi: 10.1016/j.fct.2007.11.008. Epub 2007 Nov 22.
Resveratrol, a polyphenolic phytoalexin, has free-radical scavenging activity and we found that it induces chromosomal aberrations, micronuclei, and sister chromatid exchanges in vitro. We synthesized its analogue 4-hydroxy-trans-stilbene (4-OH) and found that it has the same in vitro clastogenic activities as resveratrol, suggesting that the 4' hydroxy group of resveratrol is responsible for the effect. We fed resveratrol and 4-OH to young adult ICR mice at 0, 0.2, 2, or 20 ppm in their standard powder diet for 6 months and investigated the antioxidative effects. Half of each group was given 3000 ppm potassium bromate (KBrO(3)) in water for the last week to cause oxidative damage. Body weight gain tended to increase in males at 0.2 ppm resveratrol or 4-OH, and in females at 2 ppm 4-OH. Micronucleus (MN) analysis in bone marrow erythrocytes showed that the KBrO(3) tendency to induce MN was not prevented by the dietary resveratrol or 4-OH, which themselves did not induce MN under the present conditions. In this pilot study, resveratrol and 4-OH showed no obvious effect, either beneficial or adverse, at doses that are feasible in daily life for humans.
白藜芦醇是一种多酚类植物抗毒素，具有自由基清除活性，我们发现它在体外可诱导染色体畸变、微核及姐妹染色单体交换。我们合成了其类似物4-羟基反式芪（4-OH），发现它在体外具有与白藜芦醇相同的致断裂活性，这表明白藜芦醇的4'羟基是产生该效应的原因。我们将白藜芦醇和4-OH以0、0.2、2或20 ppm的浓度添加到成年ICR小鼠的标准粉状饲料中，喂养6个月，并研究其抗氧化作用。每组中有一半小鼠在最后一周饮用含3000 ppm溴酸钾（KBrO₃）的水以造成氧化损伤。在摄入0.2 ppm白藜芦醇或4-OH的雄性小鼠以及摄入2 ppm 4-OH的雌性小鼠中，体重增加有增加趋势。对骨髓红细胞进行微核（MN）分析表明，饲料中的白藜芦醇或4-OH并不能阻止KBrO₃诱导MN的趋势，在当前条件下它们自身也不会诱导MN。在这项初步研究中，对于人类日常生活中可行的剂量，白藜芦醇和4-OH未显示出明显的有益或有害作用。
