Detrich H William
Department of Biology, Northeastern University, Boston, Massachusetts 02115, USA.
Methods Cell Biol. 2008;85:219-41. doi: 10.1016/S0091-679X(08)85010-8.
The zebrafish is a compelling vertebrate model for understanding cellular processes in the context of the developing embryo and for analysis of cellular defects that lead to diseases such as cancer. Major advances in fluorescent protein technology have been, and will continue to be, combined with novel experimental strategies to explore these biological phenomena. Furthermore, fluorescent proteins can be used in the design of forward genetic and chemical modifier screens of ever increasing sophistication. Here I review three noteworthy applications of fluorescent proteins in zebrafish: (1) analysis of kinesin motor function in the cleaving zebrafish embryo, (2) determination of the roles of semaphorins in axonal guidance, and (3) creation of transgenic models of leukemia and other cancers.
斑马鱼是一种极具吸引力的脊椎动物模型，可用于在发育中的胚胎背景下理解细胞过程，以及分析导致癌症等疾病的细胞缺陷。荧光蛋白技术已经并将继续与新颖的实验策略相结合，以探索这些生物学现象。此外，荧光蛋白可用于设计日益复杂的正向遗传学和化学修饰剂筛选。在此，我将综述荧光蛋白在斑马鱼中的三个值得关注的应用：（1）分析分裂期斑马鱼胚胎中的驱动蛋白运动功能；（2）确定信号素在轴突导向中的作用；（3）创建白血病和其他癌症的转基因模型。
