Bertrand Marylène, Chabin Annie, Brack André, Westall Frances
Centre de Biophysique Moléculaire, CNRS, rue Charles Sadron, 45071 Orléans Cedex 2, France.
J Chromatogr A. 2008 Feb 8;1180(1-2):131-7. doi: 10.1016/j.chroma.2007.12.004. Epub 2007 Dec 8.
Two GC-MS methods for the enantioselective separation of the 20 proteinogenic amino acids are compared. Ethyl chloroformate and 2-chloropropanol were used to derivatize amino acid enantiomers. The diastereomers formed were separated on a non-chiral column by capillary gas chromatography. The separation performances were compared to those obtained when using non-chiral derivatization on a chiral column.
比较了两种用于对20种蛋白质氨基酸进行对映体选择性分离的气相色谱-质谱联用（GC-MS）方法。使用氯甲酸乙酯和2-氯丙醇对氨基酸对映体进行衍生化。所形成的非对映异构体通过毛细管气相色谱在非手性柱上进行分离。将分离性能与在手性柱上使用非手性衍生化时获得的性能进行了比较。
