Cy3-3-acylcholine: a fluorescent analogue of acetylcholine for single molecule detection.

作者信息

Fujimoto Kenzo, Yoshimura Yoshinaga, Ihara Makoto, Matsuda Kazuhiko, Takeuchi Yuko, Aoki Takaaki, Ide Toru

机构信息

School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1, Asahidai, Nomi, Ishikawa 923-1292, Japan.

出版信息

Bioorg Med Chem Lett. 2008 Feb 1;18(3):1106-9. doi: 10.1016/j.bmcl.2007.12.002. Epub 2007 Dec 7.

DOI:10.1016/j.bmcl.2007.12.002

PMID:18155907

Abstract

We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.

摘要