Li Ming Y, Chen Jiong, Shi Yu H
Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo City, Zhejiang Province, PR China.
Fish Shellfish Immunol. 2008 Feb;24(2):252-6. doi: 10.1016/j.fsi.2007.09.003. Epub 2007 Oct 5.
Leucocyte cell-derived chemotaxin-2 (LECT2) was originally demonstrated to have a chemotactic activity against human neutrophils in vitro. Current evidence suggests that LECT2 may be a multifunctional protein involved in cell growth, differentiation and autoimmune. A full-length cDNA clone of the LECT2 gene, 595bp in size, was isolated from the fish croceine croaker (Pseudosciaena crocea). It's 3'-UTR was much shorter (112nts) than that of trout LECT2 gene (210nts). Its deduced amino acid sequence of 151 residues had 39.7-75.5% identity to that of other animals. Phylogenetic analysis shows that croceine croaker LECT2 (pLECT2) is clustered tightly with other fish LECT2. The relationships of the different LECT2 coincided well with the evolutionary relationships of their organisms. In healthy fish, the expression levels of pLECT2 gene from different tissues were similar, while that in Vibrio alginolyticus-infected fish were significantly increased in liver and spleen comparing to those in healthy fish, and were a little higher in the other tissues.
白细胞衍生趋化因子2（LECT2）最初在体外被证明对人中性粒细胞具有趋化活性。目前的证据表明，LECT2可能是一种参与细胞生长、分化和自身免疫的多功能蛋白质。从大黄鱼（Pseudosciaena crocea）中分离出LECT2基因的全长cDNA克隆，大小为595bp。其3'-UTR比鳟鱼LECT2基因的3'-UTR（210nt）短得多（112nt）。其推导的151个残基的氨基酸序列与其他动物的氨基酸序列具有39.7-75.5%的同一性。系统发育分析表明，大黄鱼LECT2（pLECT2）与其他鱼类LECT2紧密聚类。不同LECT2之间的关系与其生物体的进化关系非常吻合。在健康鱼类中，不同组织中pLECT2基因的表达水平相似，而在溶藻弧菌感染的鱼类中，肝脏和脾脏中的pLECT2基因表达水平与健康鱼类相比显著增加，其他组织中的表达水平略高。
