Atkins Michelle E, Benfey Tillmann J
Department of Biology, University of New Brunswick, P.O. Box 4400, Fredericton, New Brunswick, Canada E3B 5A3.
Comp Biochem Physiol A Mol Integr Physiol. 2008 Feb;149(2):157-61. doi: 10.1016/j.cbpa.2007.11.004. Epub 2007 Nov 21.
The objective of this research was to determine whether triploid fish differ from diploids in their routine metabolic rates across a range of acclimation temperatures. Sibling diploids and triploids were acclimated to 12, 15 and 18 degrees C (Atlantic salmon; Salmo salar) and to 9, 12 and 15 degrees C (brook charr; Salvelinus fontinalis) prior to experimentation. Routine metabolic rates were then determined three times over a two-month period. Triploids of both species had higher metabolic rates than diploids at lower temperatures, and lower metabolic rates than diploids at higher temperatures, demonstrating that triploids have different (i.e., lower) thermal optima than diploids. This likely explains prior observations of high mortality of triploids at chronically elevated, but sub-lethal, rearing temperatures for sibling diploids.
本研究的目的是确定三倍体鱼在一系列驯化温度下的常规代谢率是否与二倍体不同。在实验前，将同胞二倍体和三倍体分别驯化至12、15和18摄氏度（大西洋鲑；鲑鱼）以及9、12和15摄氏度（溪红点鲑；溪鳟）。然后在两个月的时间内对常规代谢率进行了三次测定。在较低温度下，两个物种的三倍体代谢率均高于二倍体；在较高温度下，三倍体代谢率低于二倍体，这表明三倍体的最适温度与二倍体不同（即更低）。这可能解释了之前观察到的三倍体在同胞二倍体长期处于升高但亚致死的饲养温度下出现高死亡率的现象。
