Nguyen Duc T M, Nguyen Dung H, Lyun Hwa-La, Lee Hyang-Bok, Shin Jeong-Hyun, Kim Eun-Ki
Department of Biological Engineering, Inha University, Incheon, Korea.
J Microbiol Biotechnol. 2007 Oct;17(10):1585-90.
Although a number of melanogenesis inhibitors have recently been reported and used as cosmetic additives, none is completely satisfactory, leaving a need for novel skin-depigmenting agents. Thus, to develop a novel skin-depigmenting agent from natural sources, the inhibition of melanogenesis by Chinese plants was evaluated. A methanolic extract of Nigella glandulifera Freyn was found to inhibit the melanin synthesis of murine B16F10 melanoma cells by 43.7% and exhibited a low cytotoxicity (8.1 %) at a concentration of 100 microg/ml. Thus, to identify the melanogenesis-inhibiting mechanism, the inhibitory activity towards tyrosinase, the key enzyme of melanogenesis, was further evaluated, and the results showed inhibitory effects on the activity of intracellular tyrosinase yet not on mushroom tyrosinase. Finally, to isolate the compounds with a hypopigmenting capability, activity-guided isolation was performed, and Dioctyl phthalate identified as inhibiting melanogenesis.
尽管最近已报道了多种黑素生成抑制剂并将其用作化妆品添加剂，但没有一种是完全令人满意的，因此仍需要新型皮肤美白剂。因此，为了从天然来源开发新型皮肤美白剂，对中国植物抑制黑素生成的作用进行了评估。发现腺毛黑种草（Nigella glandulifera Freyn）的甲醇提取物可抑制小鼠B16F10黑色素瘤细胞的黑色素合成达43.7%，并且在浓度为100微克/毫升时表现出低细胞毒性（8.1%）。因此，为了确定黑素生成抑制机制，进一步评估了其对黑素生成关键酶酪氨酸酶的抑制活性，结果表明其对细胞内酪氨酸酶的活性有抑制作用，但对蘑菇酪氨酸酶没有抑制作用。最后，为了分离具有色素减退能力的化合物，进行了活性导向分离，邻苯二甲酸二辛酯被鉴定为具有抑制黑素生成的作用。
