Kim Hyun-Lee, Shin Eunkyoung, Kim Hong-Man, Go Hayoung, Roh Jaesook, Bae Jeehyeon, Lee Kangseok
Graduate School of Life Science and Biotechnology, Pochon CHA University, Seongnam, Korea.
J Microbiol Biotechnol. 2007 Oct;17(10):1708-11.
The Streptomyces coelicolor M145 genome harbors six copies of divergent rRNA operons that differ at ~0.2% and ~0.6% of the nucleotide positions in small subunit (SSU) and large subunit (LSU) rRNA genes, respectively. When these rRNA genes are expressed, a single cell may harbor three different kinds of SSU rRNA and five kinds of LSU rRNA. Primer extension analyses revealed that all of the heterogeneous rRNA molecules are expressed and assembled into ribosomes. This finding and the maintenance of the intragenomic variability of rRNA operons imply the existence of functional divergence of rRNA species in this developmentally complex microorganism.
天蓝色链霉菌M145基因组含有六个反向排列的rRNA操纵子拷贝，其小亚基（SSU）和大亚基（LSU）rRNA基因的核苷酸位置差异分别约为0.2%和0.6%。当这些rRNA基因表达时，单个细胞可能含有三种不同类型的SSU rRNA和五种类型的LSU rRNA。引物延伸分析表明，所有异质rRNA分子均被表达并组装成核糖体。这一发现以及rRNA操纵子基因组内变异性的维持意味着在这种发育复杂的微生物中存在rRNA种类的功能差异。
