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利用电子显微镜和原子力显微镜对马勃菌Calostoma的担孢子进行形态学鉴别。

Morphological discretion of basidiospores of the puffball mushroom Calostoma by electron and atomic force microscopy.

作者信息

Kim Misun, Kim Ki Woo, Jung Hack Sung

机构信息

School of Biological Sciences, Seoul National University, Seoul, Korea.

出版信息

J Microbiol Biotechnol. 2007 Oct;17(10):1721-6.

PMID:18156793
Abstract

Comparative morphology among species of the genus Calostoma, including C. cinnabarina, C. ravenelii, and C. japonicum, was investigated by scanning electron microscopy and atomic force microscopy. Spore morphology of cinnabarina and C. ravenelii showed no dramatic differences by light microcopy and scanning electron microscopy. To differentiate these species, atomic force microscopy was employed. Quantitative analysis of the surface roughness basidiospores revealed subtle differences in height fluctuation at the nanometer scale between the species of Calostoma. Basidiospores of C. cinnabarina had a relatively rougher surface than those of C. ravenelii at 2.0 x 2.0 micro m2 scan areas.

摘要

通过扫描电子显微镜和原子力显微镜研究了红鬼笔属(包括朱红鬼笔、拉氏鬼笔和日本鬼笔)物种间的比较形态学。通过光学显微镜和扫描电子显微镜观察,朱红鬼笔和拉氏鬼笔的孢子形态没有显著差异。为了区分这些物种,采用了原子力显微镜。对担孢子表面粗糙度的定量分析揭示了红鬼笔属物种之间在纳米尺度上高度波动的细微差异。在2.0×2.0μm2扫描区域,朱红鬼笔的担孢子表面比拉氏鬼笔的相对更粗糙。

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