定量结构照明相显微镜术

Quantitative structured-illumination phase microscopy.

作者信息

Pavani Sri Rama Prasanna, Libertun Ariel R, King Sharon V, Cogswell Carol J

机构信息

Micro Optical-Imaging Systems Laboratory, Department of Electrical and Computer Engineering, University of Colorado, Campus Box 425, Boulder, Colorado 80309, USA.

出版信息

Appl Opt. 2008 Jan 1;47(1):15-24. doi: 10.1364/ao.47.000015.

DOI:10.1364/ao.47.000015

PMID:18157271

Abstract

We introduce a quantitative phase imaging method for homogeneous objects with a bright field transmission microscope by using an amplitude mask and a digital processing algorithm. A known amplitude pattern is imaged on the sample plane containing a thick phase object by placing an amplitude mask in the field diaphragm of the microscope. The phase object distorts the amplitude pattern according to its optical path length (OPL) profile, and the distorted pattern is recorded in a CCD detector. A digital processing algorithm then estimates the object's quantitative OPL profile based on a closed form analytical solution, which is derived using a ray optics model for objects with small OPL gradients.

摘要

我们通过使用振幅掩膜和数字处理算法，介绍一种利用明场透射显微镜对均匀物体进行定量相成像的方法。通过将振幅掩膜放置在显微镜的视场光阑中，将一个已知的振幅图案成像在包含厚相位物体的样品平面上。相位物体根据其光程长度（OPL）分布使振幅图案发生畸变，畸变后的图案被记录在电荷耦合器件（CCD）探测器中。然后，一种数字处理算法基于一个封闭形式的解析解来估计物体的定量OPL分布，该解析解是使用针对具有小OPL梯度的物体的光线光学模型推导出来的。