Semiautomated T-bag peptide synthesis using 9-fluorenyl-methoxycarbonyl strategy and benzotriazol-1-yl-tetramethyl-uronium tetrafluoroborate activation.

Multiple peptide synthesis using the T-bag method is considerably facilitated using 9-fluorenyl-methoxycarbonyl strategy and benzotriazol-1-yl-tetramethyl-uronium tetrafluoroborate activation. Convenient protocols were achieved when semiautomation was applied. Successful syntheses of analogues of neuropeptide Y demonstrate the advantages of these improvements. Various peptides consisting of up to eighteen residues were built up with unusual amino acid residues. Useful hints are given for semiautomated multiple peptide synthesis for up to 120 different peptides.