Beck-Sickinger A G, Dürr H, Jung G
Institut für Organische Chemie, Universität Tübingen, Germany.
Pept Res. 1991 Mar-Apr;4(2):88-94.
Multiple peptide synthesis using the T-bag method is considerably facilitated using 9-fluorenyl-methoxycarbonyl strategy and benzotriazol-1-yl-tetramethyl-uronium tetrafluoroborate activation. Convenient protocols were achieved when semiautomation was applied. Successful syntheses of analogues of neuropeptide Y demonstrate the advantages of these improvements. Various peptides consisting of up to eighteen residues were built up with unusual amino acid residues. Useful hints are given for semiautomated multiple peptide synthesis for up to 120 different peptides.
使用芴甲氧羰基策略和苯并三唑-1-基-四甲基脲四氟硼酸盐活化，可显著促进采用T形袋法进行的多肽合成。应用半自动化时可实现便捷的方案。神经肽Y类似物的成功合成证明了这些改进的优势。构建了由多达18个残基组成的各种肽，并含有不寻常的氨基酸残基。为多达120种不同肽的半自动化多肽合成提供了有用的提示。
