Hayashida Kyoko, Kanda Keiichi, Oie Tomonori, Okamoto Yosihiro, Sakai Osamu, Watanabe Taiji, Ishibashi-Ueda Hatsue, Onoyama Masaaki, Tajikawa Tsutomu, Ohba Kenkichi, Yaku Hitoshi, Nakayama Yasuhide
Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka, Japan.
J Cardiovasc Nurs. 2008 Jan-Feb;23(1):61-4. doi: 10.1097/01.JCN.0000305053.50506.97.
"In body tissue architecture" technology is a practical concept of regenerative medicine that uses the living recipient body's reaction to a foreign object as a reactor for autologous tissue organization. A novel autologous valved conduit was produced by creating a specially designed conduit-mold composite and elastomeric scaffold for this unique in vivo tissue engineering.
Convex and concave plastic molds assembled with a small aperture of 500-800 microm were inserted into a microporous elastomeric conduit scaffold. The assembly was placed in a subcutaneous pocket of Japanese white rabbits for 1 month. The molds were pulled out from the edge of the harvested implant to obtain the valved conduit.
Homogenous and well-balanced trileaflet of membranous tissue was developed in the optimized aperture of molds. The valve leaflet closed and opened rapidly in synchronization with the backward and forward flow of the pulsatile flow circuit in vitro.
A tissue-engineered conduit incorporated with a functional autologous trileaflet valve was developed in an in vivo reactor by optimizing the microstructures of conduit scaffolds and newly designing the composite molds. The method holds promise for a safe, biocompatible, and economical heart valve prosthesis.
“体内组织结构”技术是再生医学的一个实用概念，它利用活体受体身体对外来物体的反应作为自体组织构建的反应器。通过为这种独特的体内组织工程创建专门设计的导管 - 模具复合材料和弹性支架，制造出了一种新型自体带瓣管道。
将组装好的、带有500 - 800微米小孔的凹凸塑料模具插入微孔弹性导管支架中。将该组件置于日本白兔的皮下袋中1个月。从收获的植入物边缘拉出模具以获得带瓣管道。
在优化的模具孔径中形成了均匀且平衡良好的三叶膜组织。在体外脉动流回路中，瓣膜小叶与向后和向前的血流同步快速开闭。
通过优化导管支架的微观结构并新设计复合模具，在体内反应器中开发出了一种结合功能性自体三叶瓣膜的组织工程管道。该方法有望用于制造安全、生物相容性好且经济的心脏瓣膜假体。
