Yuan Lan, Ahn In-Sook, Davis Paul F
Bioactivity Investigation Group, Wellington School of Medicine and Health Sciences, Wellington, New Zealand.
J Med Food. 2007 Dec;10(4):657-61. doi: 10.1089/jmf.2006.123.
We have previously reported that an ethanolic extract of dried shark muscle mixed with olive oil (shark muscle-olive oil [SMO]) has potent anti-angiogenic activity and that this extract appears to inhibit the binding of vascular endothelial growth factor (VEGF) to its receptor(s). In this study, we investigated the effects of SMO on the phosphorylation of VEGF receptor(s) in human umbilical vein endothelial cells (HUVECs). In vitro cell proliferation assays showed that SMO significantly reversed the VEGF-promoted increase in HUVEC proliferation. Western blot analysis revealed that SMO treatment markedly inhibited the VEGF-promoted tyrosine phosphorylation of VEGF receptor-2 (KDR) and VEGF receptor-1 (Flt-1) in a dose-dependent manner. These results demonstrated that SMO might interfere with or block the binding of VEGF with its receptors, and thereby inhibit the VEGF receptor(s) signal transduction pathway and so inhibit angiogenesis.
我们之前曾报道，干燥鲨鱼肉的乙醇提取物与橄榄油混合（鲨鱼肉-橄榄油[SMO]）具有强大的抗血管生成活性，且该提取物似乎能抑制血管内皮生长因子（VEGF）与其受体的结合。在本研究中，我们调查了SMO对人脐静脉内皮细胞（HUVECs）中VEGF受体磷酸化的影响。体外细胞增殖试验表明，SMO显著逆转了VEGF促进的HUVEC增殖增加。蛋白质印迹分析显示，SMO处理以剂量依赖的方式显著抑制了VEGF促进的VEGF受体2（KDR）和VEGF受体1（Flt-1）的酪氨酸磷酸化。这些结果表明，SMO可能干扰或阻断VEGF与其受体的结合，从而抑制VEGF受体信号转导途径，进而抑制血管生成。
