Kakizaki Ikuko, Itano Naoki, Kimata Koji, Hanada Katsumi, Kon Atsushi, Yamaguchi Masanori, Takahashi Teruno, Takagaki Keiichi
Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan.
Arch Biochem Biophys. 2008 Mar 1;471(1):85-93. doi: 10.1016/j.abb.2007.12.004. Epub 2007 Dec 15.
Hyaluronan controls keratinocyte proliferation and regeneration. We examined effect of UV on the expression of hyaluronan synthases (HASs) and hyaluronidases in cultured normal human newborn foreskin epidermal keratinocytes, NHEK(F). HAS3 mRNA was expressed predominantly and HAS2 mRNA expressed in lesser amounts and both were up-regulated after a single irradiation with moderate UVB but hyaluronidases was unchanged. Increased accumulation of hyaluronan in the culture medium mirrored the UVB-induced increase in the mRNA levels of HAS3 and HAS2. Unexpectedly, hyaluronan derived from UVB-irradiated and non-irradiated cells had identical size distribution. Increased expression of KGF and IL-1beta was detected just prior to the increase of HAS3 and HAS2 mRNAs after UVB irradiation. Antibody-neutralization study revealed that KGF and/or IL-1beta were at least involved in the up-regulation of HAS3 and HAS2 expressions. UVB-irradiated cells may enhance hyaluronan production to maintain homeostasis through up-regulation of HAS3 and HAS2 genes via cytokine response mechanism.
透明质酸控制角质形成细胞的增殖和再生。我们研究了紫外线对培养的正常人类新生儿包皮表皮角质形成细胞（NHEK(F)）中透明质酸合成酶（HASs）和透明质酸酶表达的影响。HAS3 mRNA 表达占主导，HAS2 mRNA 表达量较少，单次中等剂量紫外线 B 照射后两者均上调，但透明质酸酶未发生变化。培养基中透明质酸积累的增加反映了紫外线 B 诱导的 HAS3 和 HAS2 mRNA 水平的升高。出乎意料的是，来自紫外线 B 照射细胞和未照射细胞的透明质酸具有相同的大小分布。在紫外线 B 照射后 HAS3 和 HAS2 mRNA 增加之前，检测到 KGF 和 IL-1β 的表达增加。抗体中和研究表明，KGF 和/或 IL-1β 至少参与了 HAS3 和 HAS2 表达的上调。紫外线 B 照射的细胞可能通过细胞因子反应机制上调 HAS3 和 HAS2 基因，从而增强透明质酸的产生以维持体内平衡。
