Kinetic properties of sheep brain glutathione reductase.
作者信息
Acan N L, Tezcan E F
机构信息
Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
出版信息
Enzyme. 1991;45(3):121-4. doi: 10.1159/000468877.
PMID:1815946
Abstract
The kinetic properties of sheep brain glutathione reductase (GSSGR) were investigated. The enzyme showed Ping-Pong kinetics with double substrate inhibition in the forward direction. Km values for NADPH and GSSG were found to be 60.9 and 116.9 mumol/l, and Ki values were found to be 42.1 and 347.3 mumol/l, respectively. NADP+ inhibition at low fixed concentration of NADPH was mixed-type with a Ki of 281.5 mumol/l and alpha of 0.048. It is concluded that the enzyme shows a hybrid Ping-Pong-ordered branched mechanism.
摘要
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