Yoshioka Takeshi, Hagino Hiroshi, Yamasaki Daisuke, Okano Toru, Teshima Ryota
Department of Orthopedic Surgery, Faculty of Medicine, Tottori University, Yonago, Japan.
Mod Rheumatol. 2008;18(1):23-8. doi: 10.1007/s10165-007-0011-2. Epub 2007 Dec 26.
The influence of estrogen therapy on changes in arthritis and bone mineral density (BMD) was evaluated using an estrogen-replete collagen-induced arthritis (CIA) rat model. Seven-month-old female Sprague-Dawley rats (n = 30) were divided into the three groups; control (CONT), collagen sensitization (CIA), and CIA + 17beta-estradiol administration for 7 weeks (CIA + E). BMD was measured by peripheral quantitative computed tomography in the proximal tibia every 2 weeks. Eight weeks after the initial sensitization the rats were killed and histomorphometry of tibia was performed. The hind paw thickness increased with time in CIA rats and there was a significant difference between CONT and CIA at 8 weeks after the initial sensitization. Estrogen tended to make the development of arthritis milder. In CIA, BMD at metaphyseal cancellous bone began to decrease with the onset of arthritis and became significantly lower than in CONT after 8 weeks. Compared with the CIA, the deterioration in BMD was inhibited in CIA + E. Histomorphometrical parameters of bone resorption were increased in CIA compared with CONT, and those elevations were reduced by estrogen treatment, but estrogen had no effect on bone formation parameters. In conclusion, estrogen could partially suppress arthritis and bone loss in estrogen-replete rats as well as estrogen-deplete ones.
使用雌激素充足的胶原诱导性关节炎（CIA）大鼠模型评估雌激素疗法对关节炎变化和骨密度（BMD）的影响。将7个月大的雌性Sprague-Dawley大鼠（n = 30）分为三组：对照组（CONT）、胶原致敏组（CIA）和给予17β-雌二醇7周的CIA组（CIA + E）。每2周通过外周定量计算机断层扫描测量胫骨近端的骨密度。初次致敏8周后处死大鼠，并对胫骨进行组织形态计量学分析。CIA大鼠的后爪厚度随时间增加，初次致敏8周后CONT组和CIA组之间存在显著差异。雌激素倾向于使关节炎的发展更轻微。在CIA组中，干骺端松质骨的骨密度随着关节炎的发作开始降低，8周后显著低于CONT组。与CIA组相比，CIA + E组骨密度的恶化受到抑制。与CONT组相比，CIA组骨吸收的组织形态计量学参数增加，雌激素治疗可降低这些升高，但雌激素对骨形成参数没有影响。总之，雌激素可以部分抑制雌激素充足大鼠以及雌激素缺乏大鼠的关节炎和骨质流失。
