胰岛素样生长因子-1可抵消转化生长因子-β介导的体外人晶状体上皮细胞纤连蛋白增强作用。

IGF-1 counteracts TGF-beta-mediated enhancement of fibronectin for in vitro human lens epithelial cells.

作者信息

Chung So-Hyang, Jung Sun-Ah, Cho Young Jae, Lee Joon H, Kim Eung Kweon

机构信息

Department of Ophthalmology, Inje University College of Medicine, Seoul Paik Hospital, Seoul, Korea.

出版信息

Yonsei Med J. 2007 Dec 31;48(6):949-54. doi: 10.3349/ymj.2007.48.6.949.

DOI:10.3349/ymj.2007.48.6.949

PMID:18159585

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2628178/

Abstract

PURPOSE

To determine whether insulin-like growth factor (IGF-1) affects transforming growth factor (TGF-beta)- mediated fibronectin accumulation in human lens epithelial cell line (HLE B-3) cells.

MATERIALS AND METHODS

HLE B-3 cells were incubated for 24 hours with TGF-beta (10 ng/ mL), IGF-1 (10 ng/mL), or both. Expression of the fibronectin gene was determined using a real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Fibronectin levels were examined using western blot analysis and immunofluorescence staining.

RESULTS

Expression of the fibronectin gene was not different between the TGF-beta/IGF-1 treated group and the TGF-beta treated group (p= 0.116). However, western blot analysis demonstrated decreased fibronectin levels in human lens epithelial cells treated with TGF-beta and IGF-1 compared to those treated with TGF-beta only (p < 0.01). Immunofluorescence staining disclosed inhibition of TGF-beta-induced fibronectin in the presence of IGF-1.

CONCLUSION

This study suggests that IGF-1 counteracts TGF-beta-mediated fibronectin accumulation in human lens epithelial cells.

摘要

目的

确定胰岛素样生长因子（IGF-1）是否影响转化生长因子（TGF-β）介导的人晶状体上皮细胞系（HLE B-3）细胞中纤连蛋白的积累。

材料与方法

将HLE B-3细胞与TGF-β（10 ng/mL）、IGF-1（10 ng/mL）或两者一起孵育24小时。使用实时逆转录聚合酶链反应（RT-PCR）测定纤连蛋白基因的表达。使用蛋白质印迹分析和免疫荧光染色检查纤连蛋白水平。

结果

TGF-β/IGF-1处理组和TGF-β处理组之间纤连蛋白基因的表达没有差异（p = 0.116）。然而，蛋白质印迹分析表明，与仅用TGF-β处理的人晶状体上皮细胞相比，用TGF-β和IGF-1处理的细胞中纤连蛋白水平降低（p < 0.01）。免疫荧光染色显示在存在IGF-1的情况下，TGF-β诱导的纤连蛋白受到抑制。