转化生长因子β1在转基因小鼠晶状体中的特异性表达导致前囊下白内障。
Lens-specific expression of transforming growth factor beta1 in transgenic mice causes anterior subcapsular cataracts.
作者信息
Srinivasan Y, Lovicu F J, Overbeek P A
机构信息
Department of Cell Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
出版信息
J Clin Invest. 1998 Feb 1;101(3):625-34. doi: 10.1172/JCI1360.
Abstract
Transgenic mice were generated by microinjection of a construct containing a self-activating human TGF-beta1 cDNA driven by the lens-specific alphaA-crystallin promoter. Seven transgenic founder mice were generated, and four transgenic lines expressing TGF-beta1 were characterized. By postnatal day 21, mice from the four families exhibited anterior subcapsular cataracts. The lenses in these mice developed focal plaques of spindle-shaped cells that expressed alpha-smooth muscle actin, and that resembled the plaques seen in human anterior subcapsular cataracts. Transgenic mice showed additional ocular defects, including corneal opacification and structural changes in the iris and ciliary body. The corneal opacities were associated with increased exfoliation of the squamous layer of the corneal epithelium and increased DNA replication in the basal epithelium.
摘要
通过显微注射构建体来产生转基因小鼠，该构建体包含由晶状体特异性αA-晶体蛋白启动子驱动的自激活人转化生长因子β1（TGF-β1）cDNA。产生了7只转基因奠基小鼠，并对4个表达TGF-β1的转基因品系进行了表征。到出生后第21天，来自这四个家族的小鼠出现了前囊下白内障。这些小鼠的晶状体形成了表达α-平滑肌肌动蛋白的梭形细胞的局灶性斑块，类似于人类前囊下白内障中所见的斑块。转基因小鼠还表现出其他眼部缺陷，包括角膜混浊以及虹膜和睫状体的结构变化。角膜混浊与角膜上皮鳞状层的剥脱增加以及基底上皮中DNA复制增加有关。
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