Camier M, Benveniste D, Barré N, Brakch N, Cohen P
Groupe de Neurobiochimie Cellulaire et Moléculaire, URA 554 CNRS, Université P. et M. Curie, Paris, France.
Mol Cell Endocrinol. 1991 May;77(1-3):141-7. doi: 10.1016/0303-7207(91)90068-4.
Bovine corpus luteum is the site of intense production of pro-ocytocin-neurophysin mRNA at day 1 after estrus (Ivell et al. (1985) FEBS Lett. 190, 263-267) which is followed by apparent delayed production of ocytocin. Therefore it is a good model to study both the translational and post-translational production of this neuropeptide in non-hypothalamic tissues and its regulation. In order to assess if this mRNA is translated during the lag period we have analyzed the neurophysin-like species produced in this organ. As early as day 2 after estrus one neurophysin species (pI approximately 4.7) could be detected and was unequivocally identified as pro-ocytocin-neurophysin. In primary cultures of luteinizing granulosa cells, biosynthetic intermediates were characterized, i.e. ocytocin-Gly, ocytocin-Gly-Lys and ocytocin-Gly-Lys-Arg, whereas amidated, fully mature, ocytocin was undetectable. We conclude that translation of pro-ocytocin-neurophysin mRNA takes place soon after transcription and we propose that incomplete processing could be responsible for the low level of ocytocin in the early bovine corpus luteum.
牛黄体是发情后第1天促催产素-神经垂体素mRNA大量产生的部位（伊韦尔等人，（1985年）《欧洲生物化学学会联合会快报》190，263 - 267），随后催产素的产生明显延迟。因此，它是研究这种神经肽在非下丘脑组织中的翻译和翻译后产生及其调控的良好模型。为了评估在这段延迟期内这种mRNA是否会被翻译，我们分析了该器官中产生的类神经垂体素物质。早在发情后第2天，就可以检测到一种神经垂体素物质（等电点约为4.7），并明确鉴定为促催产素-神经垂体素。在黄体化颗粒细胞的原代培养中，对生物合成中间体进行了表征，即催产素-甘氨酸、催产素-甘氨酸-赖氨酸和催产素-甘氨酸-赖氨酸-精氨酸，而酰胺化的、完全成熟的催产素则未检测到。我们得出结论，促催产素-神经垂体素mRNA在转录后不久就会发生翻译，并且我们提出加工不完全可能是早期牛黄体中催产素水平较低的原因。
