Enriquez F J, Bradley-Dunlop D, Joens L
Hybridoma Technology, Arizona Health Sciences Center, Tucson.
Hybridoma. 1991 Dec;10(6):745-51. doi: 10.1089/hyb.1991.10.745.
Development of murine monoclonal antibodies to weakly immunogenic antigens was accomplished by combining both in vivo and in vitro immunizations. Following immunization of mice with Treponema hyodysenteriae outer membrane antigens, Manduca sexta apolipoproteins, and Drosophila melanogaster DNA polymerase, respectively, a significant increase in percentage of antibody-producing hybrids were identified when immune spleens were subjected to an in vitro immunization prior to fusion with SP2/0 myeloma cells. The hybrids developed, produced Abs to a T. hyodysenteriae 14 Kd carbohydrate, M. sexta apolipoproteins I, II, and III, and D. melanogaster DNA polymerase. The use of both in vivo and in vitro immunizations may increase the likelihood of generating monoclonal antibodies to weakly immunogenic antigens.
通过结合体内和体外免疫方法，成功制备了针对弱免疫原性抗原的鼠单克隆抗体。在用猪痢疾密螺旋体外膜抗原、烟草天蛾载脂蛋白和黑腹果蝇DNA聚合酶分别免疫小鼠后，当免疫脾细胞在与SP2/0骨髓瘤细胞融合之前进行体外免疫时，产生抗体的杂交瘤百分比显著增加。所产生的杂交瘤产生了针对猪痢疾密螺旋体14 Kd碳水化合物、烟草天蛾载脂蛋白I、II和III以及黑腹果蝇DNA聚合酶的抗体。体内和体外免疫方法的联合使用可能会增加产生针对弱免疫原性抗原的单克隆抗体的可能性。
