Nurse F, Dagan S, Tackney C, Goldstein N I
Department of Immunology/Monoclonal Antibodies, ImClone Systems Incorporated, New York, NY.
Hybridoma. 1991 Dec;10(6):753-60. doi: 10.1089/hyb.1991.10.753.
The trpE (or anthranilate synthetase) gene product has been used extensively as a fusion protein for the expression of a myriad of biologically active proteins. A trpE construct can be produced in high yield, is relatively resistant to proteolysis, and separates from the bulk of E. coli proteins because of its insolubility. We have isolated and characterized a monoclonal antibody against the TrpE protein for use as a detection and immunoaffinity reagent. The MAb, TRP 7.4, is highly specific for the TrpE protein and has a relative affinity of 1.0 ng. The antibody can also be used to detect TrpE constructs on Western blots. In addition, TRP 7.4 has been used to purify a TrpE-IL-6 fusion protein. These studies show the utility of this MAb as a tool for both research and protein purification.
色氨酸E（或邻氨基苯甲酸合成酶）基因产物已被广泛用作融合蛋白，用于表达多种生物活性蛋白。色氨酸E构建体可以高产率产生，相对抗蛋白水解，并且由于其不溶性而与大部分大肠杆菌蛋白分离。我们已经分离并鉴定了一种针对色氨酸E蛋白的单克隆抗体，用作检测和免疫亲和试剂。单克隆抗体TRP 7.4对色氨酸E蛋白具有高度特异性，相对亲和力为1.0纳克。该抗体还可用于在蛋白质印迹法上检测色氨酸E构建体。此外，TRP 7.4已用于纯化色氨酸E-白细胞介素-6融合蛋白。这些研究表明这种单克隆抗体作为研究和蛋白质纯化工具的实用性。
