采用衰减全反射傅里叶变换红外光谱法同时测定左旋多巴-卡比多巴片中的左旋多巴和卡比多巴。

Simultaneous determination of levodopa and carbidopa in levodopa-carbidopa tablets by ATR-FTIR spectrometry.

作者信息

Khanmohammadi Mohammadreza, Mobedi Elaheh, Garmarudi Amir Bagheri, Mobedi Hamid, Kargosha Kazem

机构信息

Department of Chemistry, Faculty of Science, Imam Khomeini International University, Qazvin, Iran.

出版信息

Pharm Dev Technol. 2007;12(6):573-80. doi: 10.1080/10837450701481249.

DOI:10.1080/10837450701481249

PMID:18161630

Abstract

A novel analytical procedure has been developed for quantitative determination of levodopa and carbidopa in aqueous binary solutions acidified by HCl and without any other sample pretreatment. The method is based partially on least squares treatment of data obtained by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectrometry in 1211-1315 cm(-1) and 1488-1550 cm(-1) spectral regions. The simple, rapid, and accurate proposed method was applied to determine levodopa and carbidopa in Levodopa-C tablets. The statistical parameters, such as R2, RSD, SEE, SECV, LOD, and recovery were evaluated. Number of factors, scans, and resolution were optimized. In this method R2 and RSD for levodopa and carbidopa were (0.9965, 1.209) and (0.9537, 0.813), respectively.

摘要

已开发出一种新型分析程序，用于在经盐酸酸化的二元水溶液中定量测定左旋多巴和卡比多巴，且无需任何其他样品预处理。该方法部分基于在1211 - 1315 cm(-1)和1488 - 1550 cm(-1)光谱区域通过衰减全反射傅里叶变换红外（ATR - FTIR）光谱法获得的数据的最小二乘法处理。所提出的简单、快速且准确的方法用于测定左旋多巴 - C片中的左旋多巴和卡比多巴。评估了诸如R2、RSD、SEE、SECV、LOD和回收率等统计参数。对因子数量、扫描次数和分辨率进行了优化。在该方法中，左旋多巴和卡比多巴的R2和RSD分别为(0.9965, 1.209)和(0.9537, 0.813)。