[正常及损伤脊髓中内源性胍丁胺的浓度：大鼠实验]
[Concentration of endogenous agmatine in normal and injured spinal cord: experiment with rats].
作者信息
Song Dian-Wen, Xing Wang-Xing, Jia Lian-Shun
机构信息
Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2007 Oct 9;87(37):2649-52.
OBJECTIVE
To detect the concentration of endogenous agmatine in normal and injured rats' spinal cord.
METHODS
Forty-two SD rats were randomized to sham-operation group and 1 hour, 4 hours, 8 hours, 24 hours, 48 hours, 72 hours after operation groups. The modified Allen's model of spinal cord injury was established. The endogenous agmatine was detected both in normal and injured rats' spinal cord by high-performance liquid chromatography (HPLC) with fluorescence detection and OPA derivatization.
RESULTS
The endogenous agmatine level in naive rats' spinal cord were (0.643 +/- 0.111) microg/g wet weight. After spinal cord injury, the concentration had a transient drop followed by significantly increase, and then decreased by degrees.
CONCLUSION
Up to now, little has been known about the endogenous agmatine levels in mammalian tissues. The results reported by different authors varied up to hundred times. According to our results and other articles, the endogenous agmatine levels in mammals' central nervous system arranged from 0.2 microg/g to 1.1 microg/g wet weight. Although there was a significant increase after spinal cord injury, the peak concentration was extremely lower than what it needed to take its neuroprotective effect in vitro.
目的
检测正常及脊髓损伤大鼠脊髓内内源性胍丁胺的浓度。
方法
将42只SD大鼠随机分为假手术组以及术后1小时、4小时、8小时、24小时、48小时、72小时组。建立改良的Allen脊髓损伤模型。采用高效液相色谱法（HPLC）结合荧光检测和OPA衍生化法检测正常及脊髓损伤大鼠脊髓内的内源性胍丁胺。
结果
正常大鼠脊髓内源性胍丁胺水平为（0.643±0.111）μg/g湿重。脊髓损伤后，其浓度先短暂下降，随后显著升高，然后逐渐降低。
结论
目前，关于哺乳动物组织内源性胍丁胺水平的了解甚少。不同作者报道的结果相差高达百倍。根据我们的结果及其他文献，哺乳动物中枢神经系统内源性胍丁胺水平在0.2μg/g至1.1μg/g湿重之间。虽然脊髓损伤后有显著升高，但其峰值浓度远低于其在体外发挥神经保护作用所需的浓度。
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