Wang Xianghua, Chen Weiping, Liu Wangmi, Wu Jiayan, Shao Yanqi, Zhang Xiaoming
Department of Orthopedics, The Second Affiliated Hospital School of Medicine, Zhejiang University, Hangzhou, China.
J Clin Neurosci. 2009 Jun;16(6):818-21. doi: 10.1016/j.jocn.2008.09.014. Epub 2009 Apr 1.
Spinal cord injury (SCI) continues to result in high morbidity and mortality throughout the world. An effective neuroprotective agent is still not available to counteract secondary damage caused by traumatic injury. Thrombospondin-1 (TSP-1) and transforming growth factor-beta (TGF-beta) have a role in angiogenesis, scar deposition, inflammation and may affect astrocyte phenotype and mobility. We investigated the role of TSP-1 and TGF-beta in a model of spinal cord injury in rats. Forty female Sprague-Dawley rats were randomly divided into two equal groups: the experimental group was subject to SCI using an impactor and the sham-operated group was not subject to SCI. These animals were sacrificed at 12 h and 24 h after SCI for immunochemistry and Western blot analysis of the injured spinal segment for the expression of the TSP-1 and TGF-beta proteins. We found that TSP-1 and TGF-beta expression increased immediately after SCI in the injured segment. After 12 h, TSP-1 concentrations increased more rapidly and dramatically than TGF-beta in the injured segment of the spinal cord. Elevations in TSP-1 and TGF-beta concentrations persisted for 24 h after injury. These results show that elevated expression of TSP-1 and TGF-beta can be detected in the injured segment of the spinal cord 12 and 24 h after injury. Thus, TSP-1 and TGF-beta may have a role in SCI.
脊髓损伤（SCI）在全球范围内仍然导致高发病率和高死亡率。目前仍没有有效的神经保护剂来对抗创伤性损伤引起的继发性损伤。血小板反应蛋白-1（TSP-1）和转化生长因子-β（TGF-β）在血管生成、瘢痕沉积、炎症中发挥作用，并且可能影响星形胶质细胞的表型和迁移。我们在大鼠脊髓损伤模型中研究了TSP-1和TGF-β的作用。40只雌性Sprague-Dawley大鼠被随机分为两组，每组数量相等：实验组使用撞击器造成脊髓损伤，假手术组未进行脊髓损伤。这些动物在脊髓损伤后12小时和24小时处死，对损伤的脊髓节段进行免疫化学和蛋白质印迹分析，以检测TSP-1和TGF-β蛋白的表达。我们发现，脊髓损伤后，损伤节段中TSP-1和TGF-β的表达立即增加。12小时后，脊髓损伤节段中TSP-1浓度的增加比TGF-β更快、更显著。损伤后24小时内，TSP-1和TGF-β浓度持续升高。这些结果表明，在损伤后12小时和24小时，可以在脊髓损伤节段检测到TSP-1和TGF-β的表达升高。因此，TSP-1和TGF-β可能在脊髓损伤中发挥作用。
