Soler-Argilaga C, Danon A, Wilcox H G, Heimberg M
Lipids. 1976 Jul;11(7):517-25. doi: 10.1007/BF02532896.
The concentration and composition of the very low density lipoprotein (VLDL) lipids and the behavior of the VLDL in a density gradient in the zonal ultracentrifuge were examined in plasma obtained from normal fed male and female rats before and after intravenous injection of Triton WR-1339. Concentration of lipids in plasma VLDL of female rats was about half that of male animals. Following injection with Triton WR-1339, the concentration of VLDL lipids was higher in female rats (triacylglycerol) or similar (phospholipid, cholesterol, and cholesteryl esters) in both sexes. Female rats secreted much more VLDL traicylglycerol into the plasma compartment than did the male animals under the same experimental conditions. No differences were observed in lipid composition of the VLDL or in the position of the VLDL in the zonal rotor after ultracentrifugation in a density gradient of the lipoprotein from plasma of normal male and female rats before treatment with the detergent. However, after treatment with Triton, a higher proportion of the VLDL particles isolated from plasma of female rats displayed a more rapid rate-zonal flotation in the ultracentrifuge than did the VLDL produced by the male. The VLDL secreted by female rats contained fewer moles of phospholipid and free sterol per mol triacylglycerol than did the VLDL secreted by male animals under identical experimental conditions. The molar ratio of free cholesterol:cholesteryl ester in the VLDL secreted after treatment with Trition increased in both male and female rats. Simultaneously, the content of arachidonic acid in phospholipid of VLDL increased with a concomitant decrease in cholesteryl ester. These changes in fatty acid composition suggest that the inhibitory effect of Triton on lecithin-cholesterol acyl transferase activity affects the exchange of lipids between VLDL and high density lipoprotein. It can be concluded from the data reported here that sex influences the concentration of plasma lipids in vivo and the output and properties of the VLDL.
在静脉注射曲拉通WR - 1339前后，对正常进食的雄性和雌性大鼠血浆中极低密度脂蛋白（VLDL）脂质的浓度和组成以及VLDL在区带超速离心机密度梯度中的行为进行了研究。雌性大鼠血浆VLDL中的脂质浓度约为雄性动物的一半。注射曲拉通WR - 1339后，雌性大鼠的VLDL脂质浓度更高（三酰甘油），或在两性中相似（磷脂、胆固醇和胆固醇酯）。在相同实验条件下，雌性大鼠比雄性动物向血浆区室分泌更多的VLDL三酰甘油。在用去污剂处理之前，正常雄性和雌性大鼠血浆脂蛋白在密度梯度中超速离心后，VLDL的脂质组成或VLDL在区带转子中的位置没有差异。然而，在用曲拉通处理后，从雌性大鼠血浆中分离出的VLDL颗粒在超速离心机中显示出比雄性产生的VLDL更快的速率区带漂浮。在相同实验条件下，雌性大鼠分泌的VLDL每摩尔三酰甘油所含的磷脂和游离固醇摩尔数比雄性动物分泌的VLDL少。用曲拉通处理后分泌的VLDL中游离胆固醇与胆固醇酯的摩尔比在雄性和雌性大鼠中均增加。同时，VLDL磷脂中花生四烯酸的含量增加，胆固醇酯含量随之减少。脂肪酸组成的这些变化表明，曲拉通对卵磷脂胆固醇酰基转移酶活性的抑制作用影响了VLDL与高密度脂蛋白之间的脂质交换。从这里报道的数据可以得出结论，性别影响体内血浆脂质的浓度以及VLDL的输出和性质。
