Tuominen Ilona, Pollari Maija, Aguirre von Wobeser Eneas, Tyystjärvi Esa, Ibelings Bas W, Matthijs Hans C P, Tyystjärvi Taina
Plant Physiology and Molecular Biology, Department of Biology, University of Turku, Turku, Finland.
FEBS Lett. 2008 Jan 23;582(2):346-50. doi: 10.1016/j.febslet.2007.12.030. Epub 2007 Dec 31.
The role of the primary-like sigma factor SigC was studied in Synechocystis. Under high temperature stress (48 degrees C) the DeltasigC inactivation strain showed a lower survival rate than the control strain. The DeltasigC strain grew poorly at 43 degrees C in liquid cultures under normal air. However, change to 3% CO(2) enhanced growth of DeltasigC at 43 degrees C. Differences in expression of many genes related to the carbon concentrating mechanisms between the control and the DeltasigC strain were recorded with a genome-wide DNA microarray. We suggest that low solubility of CO2 at high temperature is one of the factors contributing to the poor thermotolerance of the DeltasigC strain.
在集胞藻中研究了类原初σ因子SigC的作用。在高温胁迫（48℃）下，ΔsigC失活菌株的存活率低于对照菌株。在正常空气中，ΔsigC菌株在43℃的液体培养中生长不佳。然而，将培养环境改为3% CO₂可增强ΔsigC菌株在43℃时的生长。通过全基因组DNA微阵列记录了对照菌株和ΔsigC菌株之间许多与碳浓缩机制相关基因的表达差异。我们认为高温下CO₂的低溶解度是导致ΔsigC菌株耐热性差的因素之一。
