Inoue Shigeki, Motoda Hirotoshi, Koike Yoshihisa, Kawamura Kenji, Hiragami Fukumi, Kano Yoshio
Department of Physical Therapy, School of Health Science, Kibi International University, Takahashi, Okayama 716-8508, Japan.
Neurosci Lett. 2008 Feb 13;432(1):35-9. doi: 10.1016/j.neulet.2007.12.002. Epub 2007 Dec 8.
The increasing use of mobile phone communication has raised concerns about possible health hazard effects of microwave irradiation. We investigated damage and differentiation caused by microwave irradiation on drug-hypersensitive PC12 cell line (PC12m3). These cells showed enhancement of neurite outgrowth to various stimulants. The frequency of neurite outgrowth induced by 2.45 GHz (200 W) of microwave irradiation was approximately 10-fold greater than that of non-irradiated control cells. Incubation of PC12m3 cells with SB203580, a specific inhibitor of p38 MAPK, resulted in marked inhibition of the microwave radiation-induced neurite outgrowth. Also, activation of the transcription factor CREB induced by microwave irradiation was inhibited by SB203580. Heat shock treatment at 45 degrees C had a strong toxic effect on PC12m3 cells, whereas microwave treatment had no toxic effect on PC12m3 cells. These findings indicate that p38 MAPK is responsible for the survival of PC12m3 cells and might induce neurite outgrowth via a CREB signaling pathway when subjected to microwave irradiation.
手机通信使用的日益增加引发了人们对微波辐射可能产生的健康危害影响的担忧。我们研究了微波辐射对药物超敏PC12细胞系（PC12m3）造成的损伤和分化情况。这些细胞对各种刺激物表现出神经突生长增强。2.45 GHz（200 W）微波辐射诱导的神经突生长频率比未辐照的对照细胞高出约10倍。用p38丝裂原活化蛋白激酶（p38 MAPK）的特异性抑制剂SB203580处理PC12m3细胞，可显著抑制微波辐射诱导的神经突生长。此外，SB203580可抑制微波辐射诱导的转录因子CREB的激活。45℃热休克处理对PC12m3细胞有强烈的毒性作用，而微波处理对PC12m3细胞没有毒性作用。这些发现表明，p38 MAPK对PC12m3细胞的存活至关重要，并且在受到微波辐射时可能通过CREB信号通路诱导神经突生长。
