Yeh John, Kim Beom Su, Liang Yuan Jing, Peresie Jennifer
Department of Gynecology-Obstetrics, State University of New York at Buffalo, Buffalo, NY 14222, USA.
Am J Obstet Gynecol. 2008 Jan;198(1):82.e1-6. doi: 10.1016/j.ajog.2007.06.001.
Chemotherapeutic agents, such as cisplatin, injure the ovary. It was hypothesized that serum inhibins can serve as biomarkers of ovarian damage from cisplatin.
Adult female rats were administered cisplatin and afterward pregnant mare serum gonadotropin (PMSG) was given to stimulate ovarian inhibin production.
At baseline, the serum inhibin A levels in the cisplatin-treated groups were slightly lower than saline. However, after PMSG stimulation, the cisplatin groups had dose-related and significantly blunted serum inhibin A and B responses. Results of Western blot and enzyme-linked immunosorbent assay inhibin analysis of ovarian lysate were consistent with the serum levels. Investigation of the ovaries showed that cisplatin groups at baseline had higher percentages of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling-positive follicles in inhibin positive follicles, suggesting that cisplatin induced apoptosis in the inhibin follicles.
We conclude that serum inhibin A and B after PMSG ovarian challenge can be used as biomarkers to define rat ovarian damage after exposure to cisplatin.
顺铂等化疗药物会损伤卵巢。据推测，血清抑制素可作为顺铂所致卵巢损伤的生物标志物。
对成年雌性大鼠给予顺铂，之后给予孕马血清促性腺激素（PMSG）以刺激卵巢抑制素的产生。
在基线时，顺铂治疗组的血清抑制素A水平略低于生理盐水组。然而，在PMSG刺激后，顺铂组的血清抑制素A和B反应呈剂量相关且明显减弱。卵巢裂解物的蛋白质免疫印迹和酶联免疫吸附测定抑制素分析结果与血清水平一致。对卵巢的研究表明，基线时顺铂组抑制素阳性卵泡中末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记阳性卵泡的百分比更高，这表明顺铂诱导了抑制素卵泡的凋亡。
我们得出结论，PMSG刺激卵巢后血清抑制素A和B可作为生物标志物，用于确定大鼠暴露于顺铂后的卵巢损伤。
