Purwosunu Yuditiya, Sekizawa Akihiko, Farina Antonio, Wibowo Noroyono, Koide Keiko, Okazaki Shiho, Nakamura Masamitsu, Okai Takashi
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
Am J Obstet Gynecol. 2008 Jan;198(1):124.e1-7. doi: 10.1016/j.ajog.2007.06.079.
Placental messenger ribonucleic acid (mRNA) has been shown to circulate in maternal plasma. We investigated concentrations of vascular endothelial growth factor (VEGF), VEGF receptor-1 (VEGFR-1), and endoglin in subjects with preeclampsia, compared with normal pregnancies.
Peripheral blood samples were obtained from preeclampsia (n = 43) and control subjects (n = 41). Plasma ribonucleic acid was subjected to analysis by reverse transcription-polymerase chain reaction assay to examine the mRNA distribution among women with preeclampsia and control subjects during weeks 35-41 of gestation.
Concentrations of VEGF, VEGFR-1, and endoglin mRNA of women with preeclampsia were significantly increased. The mRNA values were observed to correlate directly with the severity of hypertension and proteinuria. VEGFR-1 mRNA was markedly elevated in women with preeclampsia and hemolysis, elevated liver enzymes, and low platelet syndrome.
The mRNA concentrations of VEGF, VEGFR-1, and endoglin were observed to correlate directly with the severity of preeclampsia.
胎盘信使核糖核酸（mRNA）已被证明可在母体血浆中循环。我们研究了子痫前期患者与正常妊娠患者相比，血管内皮生长因子（VEGF）、VEGF受体-1（VEGFR-1）和内皮糖蛋白的浓度。
从子痫前期患者（n = 43）和对照受试者（n = 41）获取外周血样本。通过逆转录-聚合酶链反应分析对血浆核糖核酸进行分析，以检查妊娠第35 - 41周子痫前期患者和对照受试者中mRNA的分布情况。
子痫前期患者的VEGF、VEGFR-1和内皮糖蛋白mRNA浓度显著升高。观察到mRNA值与高血压和蛋白尿的严重程度直接相关。子痫前期合并溶血、肝酶升高和血小板减少综合征的患者VEGFR-1 mRNA明显升高。
观察到VEGF、VEGFR-1和内皮糖蛋白的mRNA浓度与子痫前期的严重程度直接相关。
