通过分析血管生成因子的游离信使核糖核酸浓度评估胎盘的生理变化。
Evaluation of physiological alterations of the placenta through analysis of cell-free messenger ribonucleic acid concentrations of angiogenic factors.
作者信息
Purwosunu Yuditiya, Sekizawa Akihiko, Farina Antonio, Wibowo Noroyono, Koide Keiko, Okazaki Shiho, Nakamura Masamitsu, Okai Takashi
机构信息
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
出版信息
Am J Obstet Gynecol. 2008 Jan;198(1):124.e1-7. doi: 10.1016/j.ajog.2007.06.079.
OBJECTIVE
Placental messenger ribonucleic acid (mRNA) has been shown to circulate in maternal plasma. We investigated concentrations of vascular endothelial growth factor (VEGF), VEGF receptor-1 (VEGFR-1), and endoglin in subjects with preeclampsia, compared with normal pregnancies.
STUDY DESIGN
Peripheral blood samples were obtained from preeclampsia (n = 43) and control subjects (n = 41). Plasma ribonucleic acid was subjected to analysis by reverse transcription-polymerase chain reaction assay to examine the mRNA distribution among women with preeclampsia and control subjects during weeks 35-41 of gestation.
RESULTS
Concentrations of VEGF, VEGFR-1, and endoglin mRNA of women with preeclampsia were significantly increased. The mRNA values were observed to correlate directly with the severity of hypertension and proteinuria. VEGFR-1 mRNA was markedly elevated in women with preeclampsia and hemolysis, elevated liver enzymes, and low platelet syndrome.
CONCLUSION
The mRNA concentrations of VEGF, VEGFR-1, and endoglin were observed to correlate directly with the severity of preeclampsia.
目的
胎盘信使核糖核酸（mRNA）已被证明可在母体血浆中循环。我们研究了子痫前期患者与正常妊娠患者相比，血管内皮生长因子（VEGF）、VEGF受体-1（VEGFR-1）和内皮糖蛋白的浓度。
研究设计
从子痫前期患者（n = 43）和对照受试者（n = 41）获取外周血样本。通过逆转录-聚合酶链反应分析对血浆核糖核酸进行分析，以检查妊娠第35 - 41周子痫前期患者和对照受试者中mRNA的分布情况。
结果
子痫前期患者的VEGF、VEGFR-1和内皮糖蛋白mRNA浓度显著升高。观察到mRNA值与高血压和蛋白尿的严重程度直接相关。子痫前期合并溶血、肝酶升高和血小板减少综合征的患者VEGFR-1 mRNA明显升高。
结论
观察到VEGF、VEGFR-1和内皮糖蛋白的mRNA浓度与子痫前期的严重程度直接相关。
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