通过含唾液酸的粘蛋白寡糖二甲基酰胺的高温气相色谱-质谱法分析猪小肠中的含唾液酸粘蛋白寡糖。

Analysis of sialic acid-containing mucin oligosaccharides from porcine small intestine by high-temperature gas chromatography-mass spectrometry of their dimethylamides.

作者信息

Hansson G C, Bouhours J F, Karlsson H, Carlstedt I

机构信息

Department of Medical Biochemistry, University of Göteborg, Sweden.

出版信息

Carbohydr Res. 1991 Dec 16;221:179-89. doi: 10.1016/0008-6215(91)80055-r.

DOI:10.1016/0008-6215(91)80055-r

PMID:1816918

Abstract

Acetylation of sialic acid-containing oligosaccharides lactonises the sialic acid residue quantitatively for all oligosaccharides studied except for 6'-sialyl-lactose. The modified, unsulphated, sialylated and sulphated oligosaccharides can then be fractionated by anion-exchange chromatography. Ammonolysis of the lactones followed by methylation yielded the dimethylamides, which are amenable to g.l.c.-m.s. and give intense and informative mass spectra. This approach has been used to characterise the sialic acid-containing O-linked oligosaccharides obtained from the mucin glycopeptides of the small intestine of the pig. At least 28 structures were found, having NeuAc or NeuGc 6-linked to the HexNAc attached to the peptide core or to a Hex 3-linked to HexNAc. Four different disialylated oligosaccharides were found having NeuAc or NeuGc on the Hex residue 3-linked to HexNAc and 6-linked to HexNAc.

摘要

除6'-唾液酸乳糖外，含唾液酸的寡糖乙酰化可使所有研究的寡糖中的唾液酸残基定量内酯化。然后，经修饰的、未硫酸化的、唾液酸化和硫酸化的寡糖可通过阴离子交换色谱进行分离。内酯氨解后进行甲基化得到二甲基酰胺，其适用于气相色谱-质谱联用，并能给出强烈且信息丰富的质谱图。该方法已用于表征从猪小肠粘蛋白糖肽中获得的含唾液酸的O-连接寡糖。发现至少有28种结构，其中NeuAc或NeuGc以6-连接方式连接到连接在肽核心上的己糖胺或连接到以3-连接方式连接到己糖胺的己糖上。发现有四种不同的双唾液酸化寡糖，其在以3-连接方式连接到己糖胺的己糖残基上和以6-连接方式连接到己糖胺的己糖残基上含有NeuAc或NeuGc。