Drahota Z, Houstĕk J
Biochim Biophys Acta. 1977 Jun 9;460(3):541-8. doi: 10.1016/0005-2728(77)90093-7.
A simple method for isolation of adenosine triphosphatase (EC 3.6.1.3) from mitochondria is described. The enzyme is released from mitochondrial Lubrol particles by drastic sonication and purified by gel filtration on Sepharose 6-B. The described procedure is effective in isolating adenosine triphosphatase from rat liver as it is from beef heart mitochondria. The enzyme isolated from beef heart has a specific activity of 120 mumol P/min per mg protein and enzyme isolated from rat liver has a specific activity of 70 mumol P/min per mg protein when measured as a release of inorganic phosphate.
本文描述了一种从线粒体中分离三磷酸腺苷酶（EC 3.6.1.3）的简单方法。通过剧烈超声处理，该酶从线粒体的十二烷基硫醇颗粒中释放出来，并通过Sepharose 6 - B凝胶过滤进行纯化。所描述的方法从大鼠肝脏中分离三磷酸腺苷酶的效果与从牛心线粒体中分离的效果相同。当以无机磷酸盐的释放量来衡量时，从牛心中分离出的该酶的比活性为每毫克蛋白质每分钟120微摩尔磷，从大鼠肝脏中分离出的酶的比活性为每毫克蛋白质每分钟70微摩尔磷。
