Baylis S A
Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, UK.
Vox Sang. 2008 Jan;94(1):74-80. doi: 10.1111/j.1423-0410.2007.00992.x.
An extraordinary meeting of the International Working Group on the Standardization of Genome Amplification Techniques for the safety testing of blood, tissues and organs for blood borne pathogens was held on 2 March 2007, at the National Institute for Biological Standards and Control. The aim of the meeting was to investigate ways to harmonize results obtained for the detection and quantification of different genotypes of parvovirus B19 (B19V) DNA by control laboratories and manufacturers of plasma derivatives. The meeting explored issues of B19V such as the classification of B19V strains, the prevalence and distribution of different genotypes, the clinical and biological significance of different genotypes, the detection of different genotypes in plasma-derived products, and their susceptibility to virus-inactivation procedures. At this meeting and through subsequent studies, high titre, high volume samples have been identified representing different genotypes of B19V, which will be evaluated by collaborative study to prepare reference panels for the purposes of assay validation.
2007年3月2日，血液、组织和器官血源性病原体安全检测基因组扩增技术标准化国际工作组特别会议在国家生物标准与控制研究所召开。会议旨在探讨如何使对照实验室和血浆衍生物制造商在检测和定量细小病毒B19（B19V）不同基因型DNA时所获结果趋于一致。会议探讨了B19V的相关问题，如B19V毒株的分类、不同基因型的流行情况和分布、不同基因型的临床和生物学意义、血浆衍生产品中不同基因型的检测，以及它们对病毒灭活程序的敏感性。在本次会议及后续研究中，已鉴定出代表B19V不同基因型的高滴度、大容量样本，将通过协作研究对其进行评估，以制备用于检测验证的参考品组。
