Kuner T, Li Y, Gee K R, Bonewald L F, Augustine G J
Department of Neurobiology, Duke University Medical Center, Box 3209, Durham, NC 27710, USA.
Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):347-52. doi: 10.1073/pnas.0707197105. Epub 2008 Jan 2.
The time at which the N-ethylmaleimide-sensitive factor (NSF) acts during synaptic vesicle (SV) trafficking was identified by time-controlled perturbation of NSF function with a photoactivatable inhibitory peptide. Photolysis of this caged peptide in the squid giant presynaptic terminal caused an abrupt (0.2 s) slowing of the kinetics of the postsynaptic current (PSC) and a more gradual (2-3 s) reduction in PSC amplitude. Based on the rapid rate of these inhibitory effects relative to the speed of SV recycling, we conclude that NSF functions in reactions that immediately precede neurotransmitter release. Our results indicate the locus of SNARE protein recycling in presynaptic terminals and reveal NSF as a potential target for rapid regulation of transmitter release.
通过用可光激活的抑制性肽对N - 乙基马来酰亚胺敏感因子(NSF)功能进行时间控制的扰动,确定了NSF在突触小泡(SV)运输过程中发挥作用的时间。在鱿鱼巨大突触前末端对这种笼形肽进行光解,导致突触后电流(PSC)动力学突然(0.2秒)减慢,以及PSC幅度更逐渐(2 - 3秒)降低。基于这些抑制作用相对于SV循环速度的快速速率,我们得出结论,NSF在紧接神经递质释放之前的反应中发挥作用。我们的结果表明了突触前末端中SNARE蛋白循环的位点,并揭示NSF是快速调节递质释放的潜在靶点。