Xu Shan, Wang Chang-song, Zhou Jia-he
Zhejiang University of TCM, Hangzhou.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Nov;27(11):1004-6.
To explore the relationship between TCM syndrome type and gastric mucosa cell proliferation related controlling gene protein in chronic atrophic gastritis (CAG).
Expressions of cell proliferation related controlling gene protein, including proliferative cell nuclear antigen (PCNA), epidermal growth factor receptor (EGFR) and c-myc, from gastric mucosa of CAG model rats with different syndrome types were measured by immunohistochemistry and the changes of them before and after TCM intervention were also analyzed by image analysis.
Protein expressions of PCNA, EGFR and c-myc in gastric mucosa of CAG model rats with different syndrome types (Pi-deficiency type, Gan-stagnation type and dampness-heat type) were different to some extent, and all of them reduced significantly after TCM intervention in the model rats of all syndrome types.
Expressions of cell proliferation related controlling gene protein in gastric mucosa of CAG model rats of different syndrome types were different to some extent, which provides a certain experimental evidence for revealing the essence of TCM syndrome type of CAG and judging the prognosis of various types.
探讨慢性萎缩性胃炎（CAG）中医证型与胃黏膜细胞增殖相关调控基因蛋白之间的关系。
采用免疫组化法检测不同证型CAG模型大鼠胃黏膜中细胞增殖相关调控基因蛋白，包括增殖细胞核抗原（PCNA）、表皮生长因子受体（EGFR）和c-myc的表达，并通过图像分析技术分析中药干预前后其表达变化。
不同证型（脾虚型、肝郁型、湿热型）CAG模型大鼠胃黏膜中PCNA、EGFR和c-myc蛋白表达均有一定差异，且各证型模型大鼠经中药干预后其表达均显著降低。
不同证型CAG模型大鼠胃黏膜中细胞增殖相关调控基因蛋白表达存在一定差异，为揭示CAG中医证型本质及判断各型预后提供了一定的实验依据。
