Anvikar Anupkumar R, Dolla Chandrakumar, Dutta Shanta, Rao Vikas G, Gadge Vijay S, Shukla Gajadhar P, Rao Savinder, Karforma Chandan
Regional Medical Research Centre for Tribals, (Indian Council of Medical Research), Jabalpur, India.
Paediatr Perinat Epidemiol. 2008 Jan;22(1):40-6. doi: 10.1111/j.1365-3016.2007.00892.x.
Five hundred and eighty preschool children belonging to tribal areas of Madhya Pradesh were followed up daily for the presence of diarrhoea for a period of 1 year. In all, 1236 episodes of diarrhoea were recorded with an average of 2.13 episodes per child per year. Stool samples were collected during 780 episodes. They were cultured to isolate Escherichia coli as well as non-E. coli enteropathogens. Ten different genes were detected to identify all diarrhoeagenic E. coli using multiplex polymerase chain reaction. Enteroaggregative E. coli was the commonest diarrhoeagenic E. coli and was isolated from 64 children, followed by enteropathogenic E. coli (27), enterotoxigenic E. coli (10) and enterohaemorrhagic E. coli (2). Other enteropathogens detected were bacteria such as Shigella sp, Vibrio cholerae and Salmonella sp, parasites such as Giardia lamblia and Entameba histolytica, and rotavirus. Most of the bacterial pathogens were multi-drug resistant. The study shows that diarrhoeagenic E. coli contribute significantly to the burden of acute diarrhoea in tribal preschool children.
对来自中央邦部落地区的580名学龄前儿童进行了为期1年的每日腹泻情况随访。总共记录了1236次腹泻发作，平均每名儿童每年2.13次发作。在780次发作期间采集了粪便样本。对样本进行培养以分离大肠杆菌和非大肠杆菌肠道病原体。使用多重聚合酶链反应检测了10种不同基因以鉴定所有致泻性大肠杆菌。肠聚集性大肠杆菌是最常见的致泻性大肠杆菌，从64名儿童中分离出来，其次是肠致病性大肠杆菌（27例）、产肠毒素性大肠杆菌（10例）和肠出血性大肠杆菌（2例）。检测到的其他肠道病原体包括志贺氏菌属、霍乱弧菌和沙门氏菌属等细菌、蓝氏贾第鞭毛虫和溶组织内阿米巴等寄生虫以及轮状病毒。大多数细菌病原体具有多重耐药性。该研究表明，致泻性大肠杆菌对部落学龄前儿童急性腹泻负担有显著影响。
