Kim S Y, Kim Y J, Hwang S H, Kim H H, Lee E Y, Chang C L
Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Republic of Korea.
Int J Tuberc Lung Dis. 2008 Jan;12(1):39-43.
An automatic acid-fast bacilli (AFB) stainer was developed to improve laboratory workflow and protect laboratory workers.
To determine the usefulness of the 7720 Aerospray AFB slide stainer for liquefied and concentrated respiratory specimens.
With 24 smear-positive specimens submitted for AFB stain and culture in a tertiary care hospital, the autostainer was evaluated to determine whether smears would be sloughed from slides during the staining process by loading distilled water instead of staining reagents. With 109 more specimens, the performance of the 7720 Aerospray was compared with manual florescence staining as a gold standard. The tested smears were prepared with Mycohold treatment.
In most cases, the slides exposed to the autostainer containing distilled water showed lower grades of AFB than those slides not exposed to the autostainer (14/24 in Mycohold-treated slides). Almost half (54/109, 49.5%) of the Mycohold-treated autostained smears had a lower grade than the same smears stained manually. Among 62 Mycohold-treated autostained smear-negative specimens, respectively eight (12.9%) and 19 (30.6%) were high and low false-negatives.
The 7720 Aerospray showed poor performance in AFB staining of concentrated sputum specimens.
开发了一种自动抗酸杆菌（AFB）染色仪，以改善实验室工作流程并保护实验室工作人员。
确定7720型气喷式AFB载玻片染色仪对液化和浓缩呼吸道标本的实用性。
在一家三级护理医院，对24份提交进行AFB染色和培养的涂片阳性标本，通过加载蒸馏水而非染色试剂来评估自动染色仪，以确定涂片在染色过程中是否会从载玻片上脱落。对于另外109份标本，将7720型气喷式染色仪的性能与作为金标准的手动荧光染色进行比较。测试涂片采用Mycohold处理制备。
在大多数情况下，暴露于装有蒸馏水的自动染色仪的载玻片显示的AFB等级低于未暴露于自动染色仪的载玻片（Mycohold处理的载玻片中为14/24）。几乎一半（54/109，49.5%）经Mycohold处理的自动染色涂片的等级低于相同涂片的手动染色等级。在62份经Mycohold处理的自动染色涂片阴性标本中，分别有8份（12.9%）和19份（30.6%）为高假阴性和低假阴性。
7720型气喷式染色仪在浓缩痰标本的AFB染色中表现不佳。
