White Jessica A, McKinney Brandon C, John Manorama C, Powers Patricia A, Kamp Timothy J, Murphy Geoffrey G
Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan 48109-0069, USA.
Learn Mem. 2008 Jan 3;15(1):1-5. doi: 10.1101/lm.773208. Print 2008 Jan.
To determine whether L-type voltage-gated calcium channels (L-VGCCs) are required for remote memory consolidation, we generated conditional knockout mice in which the L-VGCC isoform Ca(V)1.2 was postnatally deleted in the hippocampus and cortex. In the Morris water maze, both Ca(V)1.2 conditional knockout mice (Ca(V)1.2(cKO)) and control littermates displayed a marked decrease in escape latencies and performed equally well on probe trials administered during training. In distinct contrast to their performance during training, Ca(V)1.2(cKO) mice exhibited significant impairments in spatial memory when examined 30 d after training, suggesting that Ca(V)1.2 plays a critical role in consolidation of remote spatial memories.
为了确定远程记忆巩固是否需要L型电压门控钙通道（L-VGCCs），我们构建了条件性敲除小鼠，其中L-VGCC亚型Ca(V)1.2在出生后在海马体和皮质中被敲除。在莫里斯水迷宫实验中，Ca(V)1.2条件性敲除小鼠（Ca(V)1.2(cKO)）和对照同窝小鼠在训练期间的逃避潜伏期均显著缩短，并且在训练期间进行的探针试验中表现相当。与它们在训练期间的表现形成鲜明对比的是，在训练后30天进行检查时，Ca(V)1.2(cKO)小鼠在空间记忆方面表现出明显受损迹象，这表明Ca(V)1.2在远程空间记忆巩固中起关键作用。
