黄瓜花叶病毒Y株系RNA 3衍生的缺陷型RNA的特性分析

Characterization of a defective RNA derived from RNA 3 of the Y strain of cucumber mosaic virus.

作者信息

Takeshita Minoru, Matsuo Yuka, Yoshikawa Takaya, Suzuki Masashi, Furuya Naruto, Tsuchiya Kenichi, Takanami Yoichi

机构信息

Laboratory of Plant Pathology, Faculty of Agriculture, Division of Applied Genetic and Pest Management, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka, 812-8581, Japan.

出版信息

Arch Virol. 2008;153(3):579-83. doi: 10.1007/s00705-007-0026-1. Epub 2008 Jan 4.

DOI:10.1007/s00705-007-0026-1

PMID:18175038

Abstract

A defective (D) RNA 3 naturally generated from the Y strain of cucumber mosaic virus (CMV-Y) was characterized using a biologically active cDNA clone. Sequencing of the clone revealed that the D RNA 3, named D RNA 3Yalpha, was derived from CMV-Y RNA 3 and contained a 10 nt deletion in the 5' untranslated region and a 162 nt deletion within the 3a open reading frame. Co-inoculation of D RNA 3Yalpha with CMV-Y derived from in vitro transcripts facilitated propagation of CMV-Y containing D RNA 3Yalpha in Nicotiana benthamiana or Nicotiana tabacum plants. CMV-Y, however, did not produce deletion mutants upon serial mechanical passages in the plants.

摘要

利用具有生物活性的cDNA克隆对黄瓜花叶病毒Y株系（CMV-Y）天然产生的缺陷型（D）RNA 3进行了表征。对该克隆进行测序后发现，名为D RNA 3Yα的D RNA 3源自CMV-Y RNA 3，其5'非翻译区有10个核苷酸的缺失，3a开放阅读框内有162个核苷酸的缺失。将D RNA 3Yα与体外转录产生的CMV-Y共同接种，促进了含有D RNA 3Yα的CMV-Y在本氏烟草或烟草植株中的传播。然而，CMV-Y在植株中连续机械传代时并未产生缺失突变体。