Raj S K, Srivastava A, Chandra G, Singh B P
Plant Virus Laboratory, National Botanical Research Institute, Lucknow 226001, India.
Indian J Exp Biol. 2000 Jun;38(6):613-6.
Lethal necrosis or systemic stem necrosis followed by death of Nicotiana benthamiana, severe leaf deformations of N. tabacum cv. white burley and blister formations on N. tabacum cv. samsun NN symptoms were induced by experimental inoculations of CMV RNA preparations containing satellite RNA (sat-RNA). Inoculations of RNA preparations without sat-RNA did not induce that severe symptoms on these plants, only late mild mosaic was observed. It is suggested that sat-RNA of CMV isolate has a certain role for enhancing severity of symptoms in tobacco plants. Local and systemic lethal necrosis of N. benthamiana is due to sat-RNA present with genome of CMV isolate. It is the first report of lethal necrosis induced in N. benthamiana by CMV satellite.
含有卫星RNA（sat-RNA）的黄瓜花叶病毒（CMV）RNA制剂经实验接种后，本氏烟草出现致死性坏死或全株性茎坏死随后死亡，烟草品种白肋烟出现严重的叶片畸形，烟草品种三生NN出现疱状结构。接种不含sat-RNA的RNA制剂不会在这些植株上诱发如此严重的症状，仅观察到后期出现轻度花叶病。这表明CMV分离株的sat-RNA在加重烟草植株症状严重程度方面具有一定作用。本氏烟草的局部和全株致死性坏死是由于CMV分离株基因组携带的sat-RNA所致。这是关于CMV卫星RNA诱发本氏烟草致死性坏死的首次报道。
