Nocca Giuseppina, Lupi Alessandro, De Santis Fabio, Giardina Bruno, De Palma Francesco, Chimenti Claudio, Gambarini Gianluca, De Sole Pasquale
Università Cattolica del Sacro Cuore, Istituto di Biochimica e Biochimica Clinica, Rome, Italy.
Luminescence. 2008 Jan-Feb;23(1):54-7. doi: 10.1002/bio.1018.
In the last years the studies regarding the biocompatibility of dental materials investigate, in addition to the classic cytotoxic tests, the interactions between the materials and the host cells to better explain the causes of the adverse effects observed sometimes in the clinical practice. In the present study the ability of diurethane dimethacrylate (DUDMA) and 1,4-butanediol dimethacrylate (BDDMA) methacrylic monomers present in dental composite resins to alter the functionality of peripheral blood monocytes (PBMs) and polymorphonucleate cells (PMNs) was examined. These cells are involve in the biological response to materials and in the host ability to respond to bacteria. The results obtained suggest that the examined methacrylates induce a relevant decrease of PBMs oxidative burst whereas the basal ROS production is only slightly decreased. In PMNs DUDMA induces a decrease of both basal and stimulated ROS production. BDDMA, on the contrary, it does not alter total oxidative burst in presence of stimulus while induces a statistically significant decrease of basal ROS production. Moreover this monomer alters the reaction kinetics of stimulated ROS production. The reported finding seems to indicate that this molecule could be able to stabilize PMNs in resting state and maximize their stimulated activity.
在过去几年中，关于牙科材料生物相容性的研究，除了经典的细胞毒性测试外，还研究了材料与宿主细胞之间的相互作用，以更好地解释临床实践中有时观察到的不良反应的原因。在本研究中，检测了牙科复合树脂中存在的二甲基丙烯酸二urethane（DUDMA）和二甲基丙烯酸1,4-丁二醇酯（BDDMA）甲基丙烯酸单体改变外周血单核细胞（PBMs）和多形核细胞（PMNs）功能的能力。这些细胞参与对材料的生物学反应以及宿主对细菌的反应能力。获得的结果表明，所检测的甲基丙烯酸酯会导致PBMs氧化爆发的显著降低，而基础ROS产生仅略有降低。在PMNs中，DUDMA会导致基础和刺激后的ROS产生均降低。相反，BDDMA在有刺激的情况下不会改变总氧化爆发，但会导致基础ROS产生在统计学上显著降低。此外，这种单体改变了刺激后ROS产生的反应动力学。所报道的发现似乎表明，这种分子能够使PMNs处于静止状态并使其刺激活性最大化。
