[成都地区汉族人群五个短串联重复序列系统的多态性]

[Polymorphism of five short tandem repeat systems in Chinese Han population in Chengdu].

作者信息

Li Ying, Luo Hai-bo, Song Yan-hua, Chen Xiao, Long Bin, Chen Guo-di

机构信息

West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

出版信息

Fa Yi Xue Za Zhi. 2007 Oct;23(5):338-41.

PMID:18175570

Abstract

OBJECTIVE

To obtain population genetic data of loci D11S4951, D11S4957, GATA193H05, D2S2951, and D6S2421 in Han population in Chengdu area and to validate the value of their forensic application.

METHODS

Blood samples were collected in EDTA tubes from unrelated individuals. DNAs were extracted with Chelex-100 and were analyzed by PCR and horizontal PAGE followed by silver staining.

RESULTS

Alleles 7, 10, 8, 6 and 8 were found in 5 STR loci, respectively. No deviations from Hardy-Weinberg balance were observed. The heterozygosities observed were 0.743, 0.772, 0.833, 0.650 and 0.800, respectively. The chances of exclusion were 0.497, 0.549, 0.662, 0.356 and 0.599, and the discrimination powers were 0.863, 0.912, 0.947, 0.829 and 0.931.

CONCLUSION

All of the five loci studied may be useful markers for individual identification and paternity testing.

摘要

目的

获取成都地区汉族人群中D11S4951、D11S4957、GATA193H05、D2S2951和D6S2421基因座的群体遗传数据，并验证其法医学应用价值。

方法

用乙二胺四乙酸（EDTA）抗凝管采集无关个体的血样。采用Chelex-100法提取DNA，经聚合酶链反应（PCR）和水平聚丙烯酰胺凝胶电泳（PAGE）后银染分析。

结果

在5个短串联重复序列（STR）基因座中分别发现等位基因7、10、8、6和8。未观察到偏离哈迪-温伯格平衡的情况。观察到的杂合度分别为0.743、0.772、0.833、0.650和0.800。排除概率分别为0.497、0.549、0.662、0.356和0.599，鉴别力分别为0.863、0.912、0.947、0.829和0.931。