Sugihara Takafumi, Watsuji Tomo-o, Kubota Shin, Yamada Kazune, Oka Kaori, Watanabe Kiyoshi, Meguro Michiko, Sawada Emi, Yoshihara Kiyoshi, Ueda Kenji, Beppu Teruhiko
Life Science Research Center, College of Bioresource Sciences, Nihon University, Kameino, Fujisawa,Japan.
Biosci Biotechnol Biochem. 2008 Jan;72(1):204-11. doi: 10.1271/bbb.70619. Epub 2008 Jan 7.
We study the ecological distribution of a unique syntrophic bacterium, Symbiobacterium thermophilum, and related bacteria. In this study, we found that they were frequently obtained from seashells and several marine samples. Symbiobacterium also grew from sterilized oyster shells incubated undersea for 2 or 3 months on the coast of Shimoda, Shizuoka, Japan. 16S rRNA gene-based phylogeny of the clones obtained from the Symbiobacterium-positive cultures demonstrated the potential diversity of this bacterial group, which constitutes a distinct clade between Actinobacteria and Firmicutes. We successfully isolated two new Symbiobacterium strains from oyster shells. 16S rRNA gene-based phylogeny indicated that one belongs to S. thermophilum, and that the other is affiliated with a different species. We also isolated Ureibacillius spp., which showed activity supporting the growth of S. thermophilum.
我们研究了一种独特的嗜热共生菌及其相关细菌的生态分布。在本研究中，我们发现它们经常从贝壳和一些海洋样本中获得。嗜热共生菌也能从在日本静冈县下田海岸海底培养2至3个月的灭菌牡蛎壳中生长。从嗜热共生菌阳性培养物中获得的克隆基于16S rRNA基因的系统发育表明了该细菌群体的潜在多样性，它在放线菌和厚壁菌门之间构成一个独特的进化枝。我们成功地从牡蛎壳中分离出两株新的嗜热共生菌菌株。基于16S rRNA基因的系统发育表明，一株属于嗜热栖热放线菌，另一株属于不同的物种。我们还分离出了嗜碱芽孢杆菌属，其表现出支持嗜热栖热放线菌生长的活性。
