Sakai Kouji, Sakabe Genki, Tani Orie, Watanabe Yuko, Jahangir Alam, Nakamura Masayuki, Takehara Kazuaki
Laboratory of Zoonoses, School of Veterinary Medicine, Kitasato University, Towada, Aomori, Japan.
J Vet Med Sci. 2007 Dec;69(12):1307-11. doi: 10.1292/jvms.69.1307.
A field isolate of Newcastle disease virus (NDV) isolated from northern pintail (Anas acuta) in Tohoku district, northeast Japan, was characterized. Phylogenetic analysis of the fusion protein indicated that the isolate belonged to genotype I and was closely related to isolates from the Far East corresponded to the migration route for this bird species. The isolate had the typical avirulent cleavage site of the fusion protein (112)GKQGR*L(117). In addition, pathogenicity tests indicated the isolate to have avirulent characteristics. However, the isolate has been shown to cause fusion cytopathic effects and form plaques on chicken embryo fibroblasts (CEF) in the absence of trypsin. The present results suggest that the CEF-adapted NDV, which is avirulent, is circulating among waterfowl populations.
对从日本东北部东北地区针尾鸭（Anas acuta）分离出的新城疫病毒（NDV）野毒株进行了特性鉴定。融合蛋白的系统发育分析表明，该分离株属于I型，与来自远东地区的分离株密切相关，这与该鸟类物种的迁徙路线相对应。该分离株的融合蛋白具有典型的无毒裂解位点（112）GKQGR*L（117）。此外，致病性试验表明该分离株具有无毒特性。然而，已证明该分离株在没有胰蛋白酶的情况下可引起融合细胞病变效应并在鸡胚成纤维细胞（CEF）上形成蚀斑。目前的结果表明，无毒的适应CEF的NDV正在水禽种群中传播。
