Mase Masaji, Kanehira Katsushi
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
J Vet Med Sci. 2015 Aug;77(8):919-23. doi: 10.1292/jvms.14-0684. Epub 2015 Mar 22.
To understand the epidemiology of Avian paramyxovirus serotype-1 (APMV-1) in pigeons in Japan, phylogenetic analysis was comprehensively conducted based on partial fusion protein gene using isolate from the surveillance of this virus with previously known Japanese pigeon strains. This surveillance was conducted using feces obtained from domestic pigeons collected in 40 prefectures throughout Japan from June 2011 to March 2013. From a total of 1,021 samples, a single virus (APMV1/pigeon/Japan/Kanagawa/2013: JP/Kanagawa-pg/2013) was isolated. All Japanese pigeon APMV-1 strains were clustered into a single genetic lineage, which was termed VIb/1 by phylogenetic analysis based on the F gene including the sequence of the cleavage site. These APMV-1 strains were further subdivided into four subgroups identified over 4 separate timeframes: 1984-1995 (group 1), 1995-2000 (group 2), 2001-2007 (group 3) and the novel subgroup isolated in 2013 (group 4). Each subgroup has specific amino acid motifs at a cleavage site of the F protein, namely, (112)GRQKR-F(117)(except for one strain), (112)RRKKR-F(117), (112)RRQKR-F(117) and (112)RRQKR-F(117), respectively. Our data suggest that Japanese APMV-1 strains from pigeons were diverse and reinforced the possibility that there were multiple introduction routes from foreign countries into Japan.
为了解日本鸽子中禽副粘病毒1型（APMV-1）的流行病学情况，基于部分融合蛋白基因，利用该病毒监测分离株及先前已知的日本鸽子毒株进行了全面的系统发育分析。该监测使用的粪便样本取自2011年6月至2013年3月期间从日本40个都道府县采集的家鸽。在总共1021份样本中，分离出单一病毒（APMV1/鸽子/日本/神奈川/2013：JP/神奈川-pg/2013）。通过基于包括裂解位点序列在内的F基因的系统发育分析，所有日本鸽子APMV-1毒株被聚类为单一遗传谱系，称为VIb/1。这些APMV-1毒株进一步细分为在4个不同时间框架内鉴定出的4个亚组：1984-1995年（第1组）、1995-2000年（第2组）、2001-2007年（第3组）以及2013年分离出的新亚组（第4组）。每个亚组在F蛋白裂解位点具有特定的氨基酸基序，分别为(112)GRQKR-F(117)（除一个毒株外）、(112)RRKKR-F(117)、(112)RRQKR-F(117)和(112)RRQKR-F(117)。我们的数据表明，日本鸽子来源的APMV-1毒株具有多样性，并增加了从国外进入日本存在多种引入途径的可能性。
