Umeda M
Kihara Institute for Biological Research, Yokohama City University.
Hum Cell. 1991 Dec;4(4):285-90.
P3 cell lines can be grown in protein- and lipid-free synthetic medium. Using the P3 cell culture, we have shown that these cells produce autocrine growth factors and cell-substrate attachment factors. Because the cultured cells produce proteinase-inhibitor, spent medium is applicable for inactivating the action of trypsin at the time of cell passage. In addition, we have tried to cultivate various types of cells in serum-free media on the market (ASF103, ASF104 and GIT). Many cell lines can grow in these media, but inoculum dependency is observed in some cell lines. Production of monoclonal antibody by a hybridoma cell line is rather enhanced in these media. These media can be preserved at 4 degrees C or -20 degrees C for a relatively long period. These media added with EGF support the growth of Syrian hamster embryo cells at an early passage. The growth of human diploid fibroblasts in GIT medium added with EGF is a little less compared in a serum-containing medium.
P3细胞系可在无蛋白质和脂质的合成培养基中生长。通过P3细胞培养，我们已经表明这些细胞会产生自分泌生长因子和细胞-底物附着因子。由于培养的细胞会产生蛋白酶抑制剂，因此用过的培养基适用于在细胞传代时使胰蛋白酶失活。此外，我们尝试在市售的无血清培养基（ASF103、ASF104和GIT）中培养各种类型的细胞。许多细胞系可以在这些培养基中生长，但在某些细胞系中观察到接种依赖性。杂交瘤细胞系产生单克隆抗体在这些培养基中反而会增强。这些培养基可以在4℃或-20℃下保存较长时间。添加了表皮生长因子（EGF）的这些培养基支持叙利亚仓鼠胚胎细胞在早期传代时的生长。与含血清培养基相比，添加了EGF的GIT培养基中人二倍体成纤维细胞的生长略少。
