Kheyami Ali M, Areeshi Mohammed Y, Dove Winifred, Nakagomi Osamu, Cunliffe Nigel A, Anthony Hart C
Department of Medical Microbiology, The University of Liverpool, Duncan Building, Daulby Street, Liverpool, L69 3GA, United Kingdom.
Saudi Med J. 2008 Jan;29(1):90-3.
To assess the circulating rotavirus strains among hospitalized children and adults in Gizan City.
This cross-sectional study was based in 5 hospitals in the Gizan area. Stool samples were collected between November 2004 and March 2005, from sequential patients with acute, dehydrating diarrhea. Rotavirus antigen was detected in stool by enzyme-linked immunosorbent assay. The diversity of rotavirus strains was investigated using electropherotying and reverse transcription-polymerase chain reaction amplification of the VP7 and VP4 genes (G and P genotyping).
Rotavirus was detected in 54 of 454 (12%) subjects. The ages of those infected with rotavirus ranged from 15 days to 20 years, with a median age of 36 months. The highest rotavirus detection rate (24%) occurred in children aged 48-59 months. Overall, 50 (93%) of strains could be assigned both a G- and P-type; G1P[8] was the most frequently detected strain type (n=48, 89%) with one rotavirus each of G2P[4] and G9P[8].
Rotavirus strains circulating in Gizan would be well covered by current rotavirus vaccines. Rotavirus serotype G9 has been detected in Saudi Arabia for the first time. Continued surveillance of rotavirus strains is required.
评估吉赞市住院儿童和成人中轮状病毒的流行毒株。
这项横断面研究在吉赞地区的5家医院开展。于2004年11月至2005年3月期间，从患有急性脱水腹泻的连续患者中采集粪便样本。通过酶联免疫吸附测定法检测粪便中的轮状病毒抗原。使用电泳分型以及VP7和VP4基因（G和P基因分型）的逆转录-聚合酶链反应扩增来研究轮状病毒毒株的多样性。
在454名受试者中的54名（12%）检测到轮状病毒。感染轮状病毒者的年龄范围为15天至20岁，中位年龄为36个月。轮状病毒检出率最高（24%）的是48 - 59个月大的儿童。总体而言，50株（93%）毒株可同时确定G型和P型；G1P[8]是最常检测到的毒株类型（n = 48，89%），还有1株G2P[4]和1株G9P[8]。
目前的轮状病毒疫苗可很好地覆盖吉赞市流行的轮状病毒毒株。轮状病毒血清型G9首次在沙特阿拉伯被检测到。需要持续监测轮状病毒毒株。
