采用固相萃取和液相色谱法测定大鼠血清、尿液和脑组织中6-氯-3-(3-环丙基-1,2,4-恶二唑-5-基)-5-甲基-咪唑并[1,5-a]喹喔啉-4(5H)-酮的含量。

Determination of 6-chloro-3-(3-cyclopropyl 1,2,4-oxadiazol-5-yl)-5-methyl-imidazo less than 1,5-a greater than-quinoxalin-4(5h)-one in rat serum, urine and brain by solid-phase extraction and liquid chromatography.

作者信息

Zhong W Z

机构信息

Drug Metabolism Research, Upjohn Laboratories, Kalamazoo, MI 49001.

出版信息

J Pharm Biomed Anal. 1991;9(7):573-80. doi: 10.1016/0731-7085(91)80179-d.

DOI:10.1016/0731-7085(91)80179-d

PMID:1817677

Abstract

A simple, rapid, and accurate liquid chromatographic method with ultraviolet detection and solid-phase extraction is described for the quantitation of 6-chloro-3-(3-cyclopropyl 1,2,4-oxadiazol-5-yl)-5-methyl-imidazo less than 1,5-a greater than-quinoxalin-4(5h)-one (I, U-80447) in rat serum, urine and brain. Linear calibration curves were obtained in the concentration ranges of 5 ng ml-1-20 micrograms ml-1 (serum), 20 ng ml-1-20 micrograms ml-1 (urine), and 50 ng g-1-200 micrograms g-1 (brain). Intra- and inter-assay precision and accuracy were all found to be less than 10% at the three concentrations evaluated. The absolute extraction recovery each from serum, urine and brain was greater than or equal to 90%. Application of this method to the quantitation of the title compound in rat serum and brain for a pharmacokinetic study is reported.

摘要

本文描述了一种采用紫外检测和固相萃取的简单、快速且准确的液相色谱法，用于定量测定大鼠血清、尿液和脑中的6-氯-3-(3-环丙基-1,2,4-恶二唑-5-基)-5-甲基-咪唑并[1,5-a]喹喔啉-4(5H)-酮（I，U-80447）。在5 ng ml⁻¹ - 20 μg ml⁻¹（血清）、20 ng ml⁻¹ - 20 μg ml⁻¹（尿液）和50 ng g⁻¹ - 200 μg g⁻¹（脑）的浓度范围内获得了线性校准曲线。在所评估的三个浓度下，批内和批间精密度及准确度均小于10%。血清、尿液和脑的绝对提取回收率均大于或等于90%。报道了该方法在大鼠血清和脑中定量测定标题化合物用于药代动力学研究的应用。