CXCL12及其受体CXCR4的表达与食管黏膜下癌的淋巴结转移相关。

Expression of CXCL12 and its receptor CXCR4 correlates with lymph node metastasis in submucosal esophageal cancer.

作者信息

Sasaki Ken, Natsugoe Shoji, Ishigami Sumiya, Matsumoto Masataka, Okumura Hiroshi, Setoyama Tetsuro, Uchikado Yasuto, Kita Yoshiaki, Tamotsu Kiyokazu, Sakurai Toshihide, Owaki Tetsuhiro, Aikou Takashi

机构信息

Department of Surgical Oncology and Digestive Surgery, Field of Oncology, Course of Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.

出版信息

J Surg Oncol. 2008 Apr 1;97(5):433-8. doi: 10.1002/jso.20976.

DOI:10.1002/jso.20976

PMID:18176915

Abstract

BACKGROUND AND OBJECTIVES

The chemokine CXCL12 and its receptor CXCR4 are involved in cell migration, proliferation, and angiogenesis, and promote organ-specific localization of distant metastases in various carcinomas. We examined their expression and microvessel density (MVD) in submucosal esophageal squamous cell carcinoma (ESCC) and analyzed their connection to clinicopathological findings including lymph node micrometastasis (LMM).

METHODS

Eighty-six patients with submucosal ESCC underwent curative resection from 1985 to 2002. Immunohistochemical staining of CXCL12, CXCR4, and CD34 was performed with primary tumors, and staining of cytokeratin was performed with dissected lymph nodes. MVD was calculated from CD34 expression, and LMM detected by cytokeratin staining.

RESULTS

Expression of CXCL12, but not CXCR4, correlated with lymph node metastasis. There was no significant correlation between the expression of CXCL12 and/or CXCR4 and MVD. LMM was detected in 8 cases and 14 lymph nodes. CXCL12 expression and high MVD were found in tumors with lymph node metastasis including LMM. Furthermore, in the CXCR4-positive tumors, positive CXCL12 expression was more significantly correlated with lymph node metastasis and/or LMM than negative CXCL12 expression.

CONCLUSIONS

Evaluation of CXCL12 and CXCR4 expression should assist detection of lymph node metastasis including LMM in submucosal ESCC.

摘要

背景与目的

趋化因子CXCL12及其受体CXCR4参与细胞迁移、增殖和血管生成，并促进多种癌远处转移的器官特异性定位。我们检测了它们在食管黏膜下鳞状细胞癌（ESCC）中的表达及微血管密度（MVD），并分析了它们与包括淋巴结微转移（LMM）在内的临床病理结果的关联。

方法

1985年至2002年期间，86例黏膜下ESCC患者接受了根治性切除。对原发性肿瘤进行CXCL12、CXCR4和CD34的免疫组织化学染色，对切除的淋巴结进行细胞角蛋白染色。根据CD34表达计算MVD，通过细胞角蛋白染色检测LMM。

结果

CXCL12的表达与淋巴结转移相关，而CXCR4的表达与淋巴结转移无关。CXCL12和/或CXCR4的表达与MVD之间无显著相关性。8例患者和14个淋巴结检测到LMM。在包括LMM在内的有淋巴结转移的肿瘤中发现CXCL12表达和高MVD。此外，在CXCR4阳性肿瘤中，CXCL12阳性表达与淋巴结转移和/或LMM的相关性比CXCL12阴性表达更显著。