[CD59特异性肽封条对HeLa细胞凋亡相关基因表达的影响]

[Effect of peptide seals specific to CD59 on the expression of apoptosis-related genes in HeLa cells].

作者信息

Li Xian-ping, Gao Mei-hua

机构信息

Department of Immunology, Medical College of Qingdao University, Qingdao 266021, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008 Jan;24(1):20-2.

PMID:18177611

Abstract

AIM

To study the effect of peptide seals specific to CD59 on the expression of apoptosis-related survivin, caspase-3 and bax in HeLa cells and investigate the mechanism of peptide seals specific to CD59 in inducing apoptosis of HeLa cells.

METHODS

The peptide seals specific to CD59 were put into HeLa cells and HeLa cells with CD59-transfected gene, respectively. After 24 hours of interaction, the inhibitory ratio of cell proliferation was measured by MTT and the expression of survivin, caspase-3 and bax was detected by immunohistochemistry.

RESULTS

The inhibitory ratio of HeLa cells with CD59-transfected gene + peptide seal group was higher than that of HeLa cells + peptide seal group. There was a significant difference in depressing the proliferation between two groups (P < 0.01). Compared with HeLa cells + peptide seal group, the survivin expression of HeLa cells with CD59-transfected gene + peptide seal group was decreased (P < 0.05) while caspase-3 expression was increased (P < 0.05). There was no significant difference in the expression of bax in four groups.

CONCLUSION

The peptide seal specific to CD59 can down-regulate the expression of survivin, activate caspase-3 and enhance the apoptosis of HeLa cells.

摘要

目的

研究CD59特异性肽封条对HeLa细胞中凋亡相关蛋白survivin、caspase-3和bax表达的影响，探讨CD59特异性肽封条诱导HeLa细胞凋亡的机制。

方法

将CD59特异性肽封条分别作用于HeLa细胞和转染了CD59基因的HeLa细胞。作用24小时后，采用MTT法检测细胞增殖抑制率，免疫组化法检测survivin、caspase-3和bax的表达。

结果

转染CD59基因的HeLa细胞+肽封条组的细胞增殖抑制率高于HeLa细胞+肽封条组。两组在抑制细胞增殖方面有显著差异（P<0.01）。与HeLa细胞+肽封条组相比，转染CD59基因的HeLa细胞+肽封条组的survivin表达降低（P<0.05），而caspase-3表达增加（P<0.05）。四组中bax的表达无显著差异。